PMID- 16408113
OWN - NLM
STAT- MEDLINE
DCOM- 20060223
LR  - 20220318
IS  - 0085-2538 (Print)
IS  - 0085-2538 (Linking)
VI  - 69
IP  - 2
DP  - 2006 Jan
TI  - Lipoxin A4 inhibits connective tissue growth factor-induced production of 
      chemokines in rat mesangial cells.
PG  - 248-56
AB  - Connective tissue growth factor (CTGF) is involved in mitogenesis, matrix 
      production, and chemotaxis in mesenchymal cells. The effects of CTGF on the 
      production of chemokines remain unclear. The present studies investigate the 
      regulatory role of CTGF in the production of fractalkine, monocyte 
      chemoattractant protein-1 (MCP-1), and RANTES (regulated upon activation, normal 
      T cell expressed and secreted) in cultured mesangial cells of rats, and the 
      modulatory effects of lipoxin A(4) (LXA(4)) on actions of CTGF. CTGF enhanced the 
      mRNA expression and protein release of fractalkine, MCP-1, and RANTES, the 
      expression of phospho (P)-p42/44 mitogen-activated protein kinase (MAPK), 
      P-phosphoinositide 3-kinase (PI3-K), P-Akt, and activity of nuclear factor-kappaB 
      (NF-kappaB) in mesangial cells. P-p42/44 MAPK blockade inhibited the CTGF-induced 
      expression of P-p42/44 MAPK but not NF-kappaB, and partially decreased the levels 
      of the above chemokines in supernatants. P-PI3-K blockade downregulated the 
      CTGF-stimulated expression of P-PI3-K, P-Akt, and NF-kappaB but not P-p42/44 
      MAPK, and partially decreased the release of the above chemokines. NF-kappaB 
      blockade abrogated the CTGF-activated NF-kappaB and partially decreased the 
      secretion of the above chemokines. LXA(4) dose-dependently inhibited the 
      CTGF-stimulated mRNA expression and protein release of the above chemokines, and 
      the expression of P-p42/44MAPK, P-PI3-K, P-Akt, and NF-kappaB. In conclusion, 
      these results demonstrate that CTGF induces production of fractalkine, MCP-1, and 
      RANTES via the p42/44 MAPK-, PI3-K/Akt-, and NF-kappaB-dependent signal pathway, 
      and LXA(4) downregulates the above effects of CTGF on rat mesangial cells.
FAU - Wu, S-H
AU  - Wu SH
AD  - Department of Pediatrics, Central Laboratory, The First Affiliated Hospital of 
      Nanjing Medical University, Jiangsu, People's Republic of China. kad-yc@163.com
FAU - Wu, X-H
AU  - Wu XH
FAU - Lu, C
AU  - Lu C
FAU - Dong, L
AU  - Dong L
FAU - Zhou, G-P
AU  - Zhou GP
FAU - Chen, Z-Q
AU  - Chen ZQ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Kidney Int
JT  - Kidney international
JID - 0323470
RN  - 0 (CCN2 protein, rat)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CCL5)
RN  - 0 (Chemokine CX3CL1)
RN  - 0 (Chemokines)
RN  - 0 (Chemokines, CX3C)
RN  - 0 (Cx3cl1 protein, rat)
RN  - 0 (Immediate-Early Proteins)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Lipoxins)
RN  - 0 (Membrane Proteins)
RN  - 0 (NF-kappa B)
RN  - 0 (RNA, Messenger)
RN  - 0 (lipoxin A4)
RN  - 139568-91-5 (Connective Tissue Growth Factor)
RN  - 9007-49-2 (DNA)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases)
SB  - IM
MH  - Animals
MH  - Cell Movement
MH  - Cells, Cultured
MH  - Chemokine CCL2/biosynthesis
MH  - Chemokine CCL5/biosynthesis
MH  - Chemokine CX3CL1
MH  - Chemokines/*biosynthesis
MH  - Chemokines, CX3C/biosynthesis
MH  - Connective Tissue Growth Factor
MH  - DNA/metabolism
MH  - Extracellular Signal-Regulated MAP Kinases/metabolism
MH  - Glomerular Mesangium/cytology/*metabolism
MH  - Immediate-Early Proteins/*antagonists & inhibitors
MH  - Intercellular Signaling Peptides and Proteins
MH  - Lipoxins/*pharmacology
MH  - Membrane Proteins/biosynthesis
MH  - NF-kappa B/metabolism
MH  - Phosphatidylinositol 3-Kinases/metabolism
MH  - Phosphorylation
MH  - Proto-Oncogene Proteins c-akt/metabolism
MH  - RNA, Messenger/analysis
MH  - Rats
EDAT- 2006/01/13 09:00
MHDA- 2006/02/24 09:00
CRDT- 2006/01/13 09:00
PHST- 2006/01/13 09:00 [pubmed]
PHST- 2006/02/24 09:00 [medline]
PHST- 2006/01/13 09:00 [entrez]
AID - S0085-2538(15)51459-2 [pii]
AID - 10.1038/sj.ki.5000025 [doi]
PST - ppublish
SO  - Kidney Int. 2006 Jan;69(2):248-56. doi: 10.1038/sj.ki.5000025.