PMID- 16408247
OWN - NLM
STAT- MEDLINE
DCOM- 20061011
LR  - 20061115
IS  - 0001-8244 (Print)
IS  - 0001-8244 (Linking)
VI  - 36
IP  - 2
DP  - 2006 Mar
TI  - A single copy of carbonic anhydrase 2 restores wild-type circadian period to 
      carbonic anhydrase II-deficient mice.
PG  - 301-8
AB  - Carbonic anhydrase II (CA-II)-deficient mice have long circadian periods compared 
      to their siblings with normal CA-II levels. The CA-II-deficient mice differ 
      genetically from their siblings at proximal chromosome three, where the mutated 
      carbonic anhydrase 2 gene sits on a small insert of DNA from the DBA/2J strain. 
      The rest of the genome is that of the C57BL/6J strain. The goal of this study was 
      to test the hypothesis that the null mutation in carbonic anhydrase 2 and the 
      long circadian period phenotype were linked. In order to separate the effect of 
      the null mutation in carbonic anhydrase 2 from the effect of DBA/2J alleles of 
      other genes on the insert, two new lines of mice were studied. The first line, 
      Kar, was developed from a CA-II-deficient mouse that had a fortuitous 
      recombination restoring functional CA-II without affecting the rest of the DBA/2J 
      insert. The second line was generated by breeding DBA/2J mice and C57BL/6J mice 
      until they had the genomic composition of CA-II-deficient mice without the null 
      mutation. Both lines of mice had circadian periods not different from C57BL/6J 
      mice and shorter than CA-II-deficient mice. The phenotype of the new lines showed 
      that the long circadian period characteristic of the CA-II-deficient mice arises 
      when functional CA-II is absent, not when DBA/2J alleles are present on proximal 
      chromosome three.
FAU - Kernek, Kari L
AU  - Kernek KL
AD  - Program in Medical Neurobiology, Department of Psychiatry, Institute of 
      Psychiatric Research, Indiana University School of Medicine, Indianapolis, 46202, 
      USA.
FAU - Trofatter, James A
AU  - Trofatter JA
FAU - Mayeda, Aimee R
AU  - Mayeda AR
FAU - Lahiri, Debomoy K
AU  - Lahiri DK
FAU - Hofstetter, John R
AU  - Hofstetter JR
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20060112
PL  - United States
TA  - Behav Genet
JT  - Behavior genetics
JID - 0251711
RN  - EC 4.2.1.- (Carbonic Anhydrase II)
SB  - IM
MH  - Alleles
MH  - Animals
MH  - Base Sequence/genetics
MH  - Carbonic Anhydrase II/*deficiency/*genetics
MH  - Chromosomes, Mammalian/genetics
MH  - Circadian Rhythm/*genetics
MH  - Exons/genetics
MH  - Heterozygote
MH  - Homozygote
MH  - Introns/genetics
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Inbred DBA
MH  - Models, Genetic
MH  - Mutagenesis, Insertional
MH  - Phenotype
MH  - Recombination, Genetic/genetics
EDAT- 2006/01/13 09:00
MHDA- 2006/10/13 09:00
CRDT- 2006/01/13 09:00
PHST- 2005/04/20 00:00 [received]
PHST- 2005/07/14 00:00 [accepted]
PHST- 2006/01/13 09:00 [pubmed]
PHST- 2006/10/13 09:00 [medline]
PHST- 2006/01/13 09:00 [entrez]
AID - 10.1007/s10519-005-9032-9 [doi]
PST - ppublish
SO  - Behav Genet. 2006 Mar;36(2):301-8. doi: 10.1007/s10519-005-9032-9. Epub 2006 Jan 
      12.