PMID- 16410384
OWN - NLM
STAT- MEDLINE
DCOM- 20070118
LR  - 20060517
IS  - 1066-5099 (Print)
IS  - 1066-5099 (Linking)
VI  - 24
IP  - 5
DP  - 2006 May
TI  - Ciliated cells differentiated from mouse embryonic stem cells.
PG  - 1381-8
AB  - In the present study, we demonstrated that the mouse embryonic stem cells were 
      differentiated into ciliated epithelial cells, with characteristics of normal 
      ciliated cells. These cells expressed ciliary marker proteins, such as 
      beta-tubulin IV and hepatocyte nuclear factor-3/forkhead homolog 4 (HFH-4), and 
      processed microtubules were arranged in the 9 + 2 structure, which is the same 
      specific alignment observed in normal ciliary microtubules. The cilia of these 
      cells were beating at a frequency of 17-20 Hz. The differentiated embryoid bodies 
      (EBs) containing these ciliated cells expressed respiratory marker genes such as 
      thyroid transcription factor-1 and surfactant protein-C. For the induction of 
      ciliated cells, culture of EBs in serum-free medium during the initial 2 days of 
      the attachment was indispensable. When EBs were treated with bone morphogenetic 
      proteins, the expression of HFH-4 was decreased, and the ciliated cells were 
      scarcely differentiated. Previous methods for inducing ciliated cells in vitro 
      from embryonic or adult tissues involved an air-liquid interface. The system used 
      in this study more closely mimics the normal development of ciliated cells; thus, 
      an added advantage of the system is as a tool for studying the differentiation 
      mechanism of normal ciliated epithelial cells.
FAU - Nishimura, Yusuke
AU  - Nishimura Y
AD  - Department of Biological Science, The University of Tokyo, Japan.
FAU - Hamazaki, Tatsuo S
AU  - Hamazaki TS
FAU - Komazaki, Shinji
AU  - Komazaki S
FAU - Kamimura, Shinji
AU  - Kamimura S
FAU - Okochi, Hitoshi
AU  - Okochi H
FAU - Asashima, Makoto
AU  - Asashima M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060112
PL  - England
TA  - Stem Cells
JT  - Stem cells (Dayton, Ohio)
JID - 9304532
RN  - 0 (Culture Media, Serum-Free)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
SB  - IM
MH  - Animals
MH  - Cell Differentiation/drug effects/*physiology
MH  - Cilia/physiology/*ultrastructure
MH  - Culture Media, Serum-Free/pharmacology
MH  - Embryonic Stem Cells/*cytology/drug effects
MH  - Epithelial Cells/*cytology/drug effects
MH  - Intercellular Signaling Peptides and Proteins/pharmacology
MH  - Mice
EDAT- 2006/01/18 09:00
MHDA- 2007/01/19 09:00
CRDT- 2006/01/18 09:00
PHST- 2006/01/18 09:00 [pubmed]
PHST- 2007/01/19 09:00 [medline]
PHST- 2006/01/18 09:00 [entrez]
AID - 2005-0464 [pii]
AID - 10.1634/stemcells.2005-0464 [doi]
PST - ppublish
SO  - Stem Cells. 2006 May;24(5):1381-8. doi: 10.1634/stemcells.2005-0464. Epub 2006 
      Jan 12.