PMID- 16410636
OWN - NLM
STAT- MEDLINE
DCOM- 20060623
LR  - 20191026
IS  - 0951-6433 (Print)
IS  - 0951-6433 (Linking)
VI  - 23
IP  - 3
DP  - 2005
TI  - Extraction and chromatographic separation of anticarcinogenic fractions from 
      cacao bean husk.
PG  - 141-50
AB  - The utilization of cacao bean husk (CBH), a by-product of chocolate manufacture, 
      would be both environmentally and economically beneficial. For this purpose, a 
      process for effectively separating and fractionating CBH fractions having cancer 
      preventive potential was developed in this study. For screening the fractions 
      with potent cancer preventive activity, we examined the effect of extracts and 
      fractions of CBH on the inhibition of gap-junction intercellular communication 
      (GJIC) and the DNA synthesis of cancer cells, both of which are characteristics 
      of the promotion and progression stages in carcinogenesis. The extracts of CBH 
      (especially, the 60% ethanol fraction after extraction with 50% acetone) 
      containing 43 wt.% polyphenol exerted an excellent protective effect on 
      H2O2-induced inhibition of GJIC in WB-F344 rat liver epithelial cells as 
      determined by the scrape-loading/dye transfer assay. The enhancement of GJIC by 
      the extracts of CBH was approximately 10-fold higher than that of a well-known 
      dietary chemopreventive component, vitamin C. The extracts of CBH (especially, 
      the 60% ethanol fraction) also suppressed DNA synthesis in all liver, stomach, 
      and colon cancer cells as demonstrated by the ;3H-thymidine incorporation assay, 
      by approximately four-fold higher than that of vitamin C. These results imply 
      that the polyphenol extracts and fractions of CBH are effective functional 
      materials to be used in either preventing or inhibiting cancer.
FAU - Lee, Ki Won
AU  - Lee KW
AD  - Department of Food Science and Technology, School of Agricultural Biotechnology 
      and Center for Agricultural Biomaterials, Seoul National University, Seoul 
      151-742, Republic of Korea.
FAU - Hwang, Eun-Sun
AU  - Hwang ES
FAU - Kang, Nam Joo
AU  - Kang NJ
FAU - Kim, Kyoung Heon
AU  - Kim KH
FAU - Lee, Hyong Joo
AU  - Lee HJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Biofactors
JT  - BioFactors (Oxford, England)
JID - 8807441
RN  - 0 (Anticarcinogenic Agents)
RN  - 0 (DNA, Neoplasm)
RN  - 0 (Phenols)
RN  - 0 (Plant Extracts)
RN  - BBX060AN9V (Hydrogen Peroxide)
SB  - IM
MH  - Animals
MH  - Anticarcinogenic Agents/*isolation & purification/pharmacology
MH  - Cacao/*chemistry
MH  - Cell Division/drug effects
MH  - Cells, Cultured
MH  - DNA, Neoplasm/biosynthesis
MH  - Epithelial Cells/ultrastructure
MH  - Gap Junctions/drug effects
MH  - Hydrogen Peroxide/analysis/pharmacology
MH  - Liver/ultrastructure
MH  - Phenols/analysis
MH  - Plant Extracts/pharmacology
MH  - Rats
MH  - Rats, Inbred F344
MH  - Seeds/*chemistry
EDAT- 2006/01/18 09:00
MHDA- 2006/06/24 09:00
CRDT- 2006/01/18 09:00
PHST- 2006/01/18 09:00 [pubmed]
PHST- 2006/06/24 09:00 [medline]
PHST- 2006/01/18 09:00 [entrez]
AID - 10.1002/biof.5520230303 [doi]
PST - ppublish
SO  - Biofactors. 2005;23(3):141-50. doi: 10.1002/biof.5520230303.