PMID- 16411098
OWN - NLM
STAT- MEDLINE
DCOM- 20070126
LR  - 20181201
IS  - 0721-832X (Print)
IS  - 0721-832X (Linking)
VI  - 244
IP  - 9
DP  - 2006 Sep
TI  - Acute retinal necrosis diagnosed in a child with chronic panuveitis.
PG  - 1206-8
AB  - BACKGROUND: To report the case of an immunocompetent child with herpes simplex 
      virus-2 (HSV-2) acute retinal necrosis (ARN) syndrome, who was considered to have 
      an idiopathic unilateral panuveitis sensitive to steroid treatment. METHODS: 
      Polymerase chain reaction for detection of viral DNA was applied to ocular fluids 
      and in situ hybridization was performed on a retinal sample. HSV serology was 
      performed using the ELISA and Western blot techniques, and an in-house indirect 
      immunofluorescence technique. RESULTS: In addition to the atypical clinical 
      presentation, the serological assays for HSV were negative using ELISA at the 
      time of diagnosis of ARN and 1 year after. HSV2 infection was confirmed by using 
      polymerase chain reaction of aqueous humor specimen and in situ hybridization of 
      a retinal biopsy. Retrospective analysis with the Western blot technique detected 
      low titers of anti-HSV antibodies, when the sera were concentrated 5-fold. 
      CONCLUSION: Herpes virus infections must be investigated in children with 
      posterior or panuveitis. PCR analysis is a reliable technique for diagnosis. This 
      case emphasizes that clinical presentation can be atypical and that a negative 
      viral serology does not exclude an acute or a past herpetic infection.
FAU - Chiquet, Christophe
AU  - Chiquet C
AD  - Department of Ophthalmology, Hopital Michallon, CHU de Grenoble, BP217, Grenoble, 
      Cedex 09, France. cchiquet@chu-grenoble.fr
FAU - Bodaghi, Bahram
AU  - Bodaghi B
FAU - Mougin, Christiane
AU  - Mougin C
FAU - Najioullah, Fatiha
AU  - Najioullah F
LA  - eng
PT  - Case Reports
PT  - Journal Article
DEP - 20060113
PL  - Germany
TA  - Graefes Arch Clin Exp Ophthalmol
JT  - Graefe's archive for clinical and experimental ophthalmology = Albrecht von 
      Graefes Archiv fur klinische und experimentelle Ophthalmologie
JID - 8205248
RN  - 0 (Antibodies, Viral)
RN  - 0 (Antiviral Agents)
RN  - 0 (DNA, Viral)
RN  - HG18B9YRS7 (Valine)
RN  - MZ1IW7Q79D (Valacyclovir)
RN  - X4HES1O11F (Acyclovir)
SB  - IM
MH  - Acyclovir/analogs & derivatives/therapeutic use
MH  - Antibodies, Viral/blood
MH  - Antiviral Agents/therapeutic use
MH  - Aqueous Humor/virology
MH  - Child, Preschool
MH  - Chronic Disease
MH  - DNA, Viral/analysis
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Eye Infections, Viral/diagnosis/drug therapy/*virology
MH  - Herpes Simplex/diagnosis/drug therapy/*virology
MH  - Herpesvirus 2, Human/*isolation & purification
MH  - Humans
MH  - In Situ Hybridization
MH  - Male
MH  - Panuveitis/diagnosis/drug therapy/*virology
MH  - Polymerase Chain Reaction
MH  - Retinal Necrosis Syndrome, Acute/diagnosis/drug therapy/*virology
MH  - Valacyclovir
MH  - Valine/analogs & derivatives/therapeutic use
EDAT- 2006/01/18 09:00
MHDA- 2007/01/27 09:00
CRDT- 2006/01/18 09:00
PHST- 2005/09/18 00:00 [received]
PHST- 2005/12/04 00:00 [accepted]
PHST- 2005/11/16 00:00 [revised]
PHST- 2006/01/18 09:00 [pubmed]
PHST- 2007/01/27 09:00 [medline]
PHST- 2006/01/18 09:00 [entrez]
AID - 10.1007/s00417-005-0233-y [doi]
PST - ppublish
SO  - Graefes Arch Clin Exp Ophthalmol. 2006 Sep;244(9):1206-8. doi: 
      10.1007/s00417-005-0233-y. Epub 2006 Jan 13.