PMID- 16412331
OWN - NLM
STAT- MEDLINE
DCOM- 20070914
LR  - 20181203
IS  - 0529-567X (Print)
IS  - 0529-567X (Linking)
VI  - 40
IP  - 12
DP  - 2005 Dec
TI  - [RNA interference silencing expression of survivin gene and reversing drug 
      resistance of ovarian cancer cell line SKOV3/ADM].
PG  - 836-9
AB  - OBJECTIVE: To examine expression of survivin gene in ovarian epithelial carcinoma 
      drug resistant cell line SKOV3/ADM and its parental cell line SKOV3, and 
      induction of cells apoptosis and reversal of drug resistance in SKOV3/ADM after 
      RNA interference (RNAi) silencing survivin gene. METHODS: The transcription of 
      survivin gene in cells was detected by semi-quantitative RT-PCR, the protein 
      expression level of survivin gene was analyzed by immunofluorescence staining. 
      SKOV3/ADM cells were treated with pshRNA-survivin and paclitaxel (Taxol), and 
      acridine orange (AO)/ethidium bromide (EB) staining was performed to evaluate the 
      apoptosis of cells. RESULTS: Survivin gene mRNA expressed by 99.1% and 75.3% 
      respectively in cell lines SKOV3/ADM and SKOV3, while fluorescent cells were 59 
      +/- 5 and 42 +/- 3 (P < 0.05). After the introduction of pshRNA-survivin into 
      SKOV3/ADM, mRNA transcription level of survivin gene decreased distinctly from 
      99.1% to 7.9%. The apoptotic cells of control group detected by AO/EB staining 
      was 3.6 +/- 0.6, of Taxol group 10.2 +/- 1.0, of RNAi group 48.5 +/- 4.9, of RNAi 
      + Taxol group 71.5 +/- 6.8. Apoptosis ratio between RNAi + Taxol group and RNAi 
      group had significant difference (P < 0.05), and that between RNAi + Taxol group 
      and Taxol group also had significant difference (P < 0.05). CONCLUSIONS: Both 
      survivin gene mRNA and its protein are over-expressed in ovarian epithelial 
      carcinoma cell lines SKOV3 and SKOV3/ADM, the level of survivin gene expressed in 
      SKOV3/ADM is obviously different compared with that in its parental cell line 
      SKOV3. RNA interference targeted against specific sequences of survivin in 
      SKOV3/ADM cell could significantly reduce the level of survivin mRNA transcripts 
      and protein, effectively induce the cells apoptosis and restore the sensitivity 
      of cell to conventional chemotherapeutic agents Taxol.
FAU - Deng, Kai-xian
AU  - Deng KX
AD  - Department of Obstetrics and Gynecology, Second Affiliated Hospital, Chongqing 
      University of Medical Science, Chongqing 400010, China.
FAU - Zhong, Ling
AU  - Zhong L
FAU - Jiang, Mei-xian
AU  - Jiang MX
FAU - Wang, Ping-ling
AU  - Wang PL
FAU - Chen, Ying
AU  - Chen Y
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhonghua Fu Chan Ke Za Zhi
JT  - Zhonghua fu chan ke za zhi
JID - 16210370R
RN  - 0 (Antineoplastic Agents)
RN  - 0 (BIRC5 protein, human)
RN  - 0 (Inhibitor of Apoptosis Proteins)
RN  - 0 (Microtubule-Associated Proteins)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Survivin)
SB  - IM
MH  - Animals
MH  - Antineoplastic Agents/pharmacology
MH  - Apoptosis
MH  - Cell Line, Tumor
MH  - Drug Resistance, Neoplasm/*genetics
MH  - Female
MH  - *Gene Silencing
MH  - Humans
MH  - Inhibitor of Apoptosis Proteins
MH  - Microtubule-Associated Proteins/biosynthesis/*genetics
MH  - Neoplasm Proteins/*genetics
MH  - Ovarian Neoplasms/genetics/*metabolism
MH  - RNA Interference
MH  - RNA, Messenger/biosynthesis/genetics
MH  - *RNA, Small Interfering
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Survivin
MH  - Transfection
EDAT- 2006/01/18 09:00
MHDA- 2007/09/15 09:00
CRDT- 2006/01/18 09:00
PHST- 2006/01/18 09:00 [pubmed]
PHST- 2007/09/15 09:00 [medline]
PHST- 2006/01/18 09:00 [entrez]
PST - ppublish
SO  - Zhonghua Fu Chan Ke Za Zhi. 2005 Dec;40(12):836-9.