PMID- 16413396
OWN - NLM
STAT- MEDLINE
DCOM- 20060323
LR  - 20141120
IS  - 0301-472X (Print)
IS  - 0301-472X (Linking)
VI  - 34
IP  - 1
DP  - 2006 Jan
TI  - In vitro transdifferentiation of adult bone marrow Sca-1+ cKit- cells cocultured 
      with fetal liver cells into hepatic-like cells without fusion.
PG  - 97-106
AB  - Several research groups have recently reported that certain bone marrow cells 
      (BMCs) differentiate into hepatocytes in vitro as well as in vivo in rodents. 
      However, it has yet to be elucidated what factors effectively trigger and sustain 
      transdifferentiation of BMCs. In the present study, we specifically asked whether 
      the presence of murine fetal liver cells (FLCs) triggered and supported in vitro 
      transdifferentiation of murine BMCs. Fractionated BMCs from green fluorescence 
      protein (GFP)-expressing transgenic mice and FLCs from ROSA26 mice (X-gal(+) 
      FLCs) were cocultured in the presence of hepatocyte growth factor in 
      laminin-coated dishes. We found that Sca-1(+) BMCs gave rise to adherent 
      hepatic-like cells, which expressed albumin as assessed with immunocytochemistry 
      and RNA-polymerase chain reaction (PCR), and alpha-fetoprotein and cytokeratin 19 
      as examined with RNA-PCR. When GFP(+)Sca-1(+)cKit(-) cells were cocultured with 
      X-gal(+) FLCs, all GFP(+) albumin-producing cells were negative for X-gal, 
      showing that cell fusion was not associated in the observed BMCs' differentiation 
      into hepatic-like cells. Titration analysis revealed that 1 of 5,943 
      Sca-1(+)cKit(-) cells had the ability to proliferate and differentiate into 
      hepatic-like cells. These data strongly suggest that BMCs differentiate into 
      hepatic-like cells in the presence of FLCs and that the present method may be 
      useful for propagating BMC-derived hepatocytic progenitors and for investigating 
      the nature of those cells.
FAU - Yamada, Yasuhiro
AU  - Yamada Y
AD  - Department of Hematology, Kumamoto University School of Medicine, Kumamoto, 
      Japan.
FAU - Nishimoto, Eishi
AU  - Nishimoto E
FAU - Mitsuya, Hiroaki
AU  - Mitsuya H
FAU - Yonemura, Yuji
AU  - Yonemura Y
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Exp Hematol
JT  - Experimental hematology
JID - 0402313
RN  - 0 (Antigens, Ly)
RN  - 0 (Ly6a protein, mouse)
RN  - 0 (Membrane Proteins)
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - IM
MH  - Animals
MH  - Antigens, Ly/*biosynthesis
MH  - Bone Marrow Cells/*cytology/metabolism
MH  - Cell Differentiation/physiology
MH  - Coculture Techniques
MH  - Green Fluorescent Proteins/physiology
MH  - Immunohistochemistry
MH  - Liver/*cytology/embryology/physiology
MH  - Membrane Proteins/*biosynthesis
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Transgenic
EDAT- 2006/01/18 09:00
MHDA- 2006/03/24 09:00
CRDT- 2006/01/18 09:00
PHST- 2004/11/01 00:00 [received]
PHST- 2005/09/21 00:00 [revised]
PHST- 2005/09/23 00:00 [accepted]
PHST- 2006/01/18 09:00 [pubmed]
PHST- 2006/03/24 09:00 [medline]
PHST- 2006/01/18 09:00 [entrez]
AID - S0301-472X(05)00543-6 [pii]
AID - 10.1016/j.exphem.2005.09.018 [doi]
PST - ppublish
SO  - Exp Hematol. 2006 Jan;34(1):97-106. doi: 10.1016/j.exphem.2005.09.018.