PMID- 16413558
OWN - NLM
STAT- MEDLINE
DCOM- 20060526
LR  - 20131121
IS  - 0021-9673 (Print)
IS  - 0021-9673 (Linking)
VI  - 1107
IP  - 1-2
DP  - 2006 Feb 24
TI  - Determination of folic acid by capillary electrophoresis with chemiluminescence 
      detection.
PG  - 290-3
AB  - A rapid and simple method is presented for the determination of folic acid (FA) 
      by capillary electrophoresis (CE) with chemiluminescence (CL) detection. This 
      method was based on enhance effect of FA on the CL reaction between luminol and 
      BrO(-) in alkaline aqueous solution. Optimal separation and determination was 
      obtained with an electrophoretic buffer of 35 mM sodium borate (pH 9.4) 
      containing 0.8 mM luminol, and an oxidizer solution of 1.6 mM NaBrO in 100 mM 
      NaCO(3) buffer solution (pH 12.0). Under the optimal conditions, the 
      determination of FA was achieved in less than 20 min, and the detection limit was 
      2.0 x 10(-8) M (S/N=3). The relative standard deviations (RSDs) on peak area and 
      migration time were in the 1.5 and 1.1%, respectively. The present CE-CL method 
      was applied to the determination of FA in commercial pharmaceutical tablets, 
      apple juices and human urine.
FAU - Zhao, Shulin
AU  - Zhao S
AD  - College of Chemical Engineering, Sichuan University, Chengdu 610065, China.
FAU - Yuan, Hongyan
AU  - Yuan H
FAU - Xie, Chao
AU  - Xie C
FAU - Xiao, Dan
AU  - Xiao D
LA  - eng
PT  - Journal Article
DEP - 20060118
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 0 (Tablets)
RN  - 5EXP385Q4F (Luminol)
RN  - 935E97BOY8 (Folic Acid)
SB  - IM
MH  - Electrophoresis, Capillary/*methods
MH  - Folic Acid/*analysis
MH  - Luminescent Measurements
MH  - Luminol/chemistry
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Tablets/chemistry
EDAT- 2006/01/18 09:00
MHDA- 2006/05/27 09:00
CRDT- 2006/01/18 09:00
PHST- 2005/07/26 00:00 [received]
PHST- 2005/11/16 00:00 [revised]
PHST- 2005/11/17 00:00 [accepted]
PHST- 2006/01/18 09:00 [pubmed]
PHST- 2006/05/27 09:00 [medline]
PHST- 2006/01/18 09:00 [entrez]
AID - S0021-9673(05)02209-0 [pii]
AID - 10.1016/j.chroma.2005.11.052 [doi]
PST - ppublish
SO  - J Chromatogr A. 2006 Feb 24;1107(1-2):290-3. doi: 10.1016/j.chroma.2005.11.052. 
      Epub 2006 Jan 18.