PMID- 1641776
OWN - NLM
STAT- MEDLINE
DCOM- 19920828
LR  - 20100324
IS  - 0039-6060 (Print)
IS  - 0039-6060 (Linking)
VI  - 112
IP  - 2
DP  - 1992 Aug
TI  - Induction of donor-specific tolerance to rat cardiac allografts by intrathymic 
      inoculation of bone marrow.
PG  - 370-6; discussion 376-7
AB  - BACKGROUND: Induction of donor-specific tolerance to tissue or organ allografts 
      can readily be achieved by administration of allogeneic bone marrow to neonatal 
      rodents; however, in adult recipients induction of transplantation tolerance by 
      this strategy generally requires intensive cytoablative conditioning. Described 
      here is a novel method of promoting transplantation tolerance that involves 
      inoculation of donor bone marrow into the thymus of transiently immunosuppressed 
      adult recipients. METHODS: Prospective Wistar-Furth recipients were inoculated 
      with allogeneic Lewis bone marrow cells (BMCs) either intrathymically or 
      intravenously in conjunction with a single dose of antilymphocyte serum 2 to 3 
      weeks before receiving donor-strain cardiac allografts. Recipients were monitored 
      for graft survival and examined for presence of hematopoietic chimerism. RESULTS: 
      Intrathymic but not intravenous inoculation of donor BMCs led to permanent 
      survival of donor-strain cardiac allografts, whereas third-party Dark agouti 
      cardiac allografts were rejected promptly. Persistence of donor chimerism was 
      demonstrated in the thymus of Wistar-Furth recipients of intrathymic Lewis BMCs 
      for as long as 3 weeks after BMC inoculation. CONCLUSIONS: Intrathymic 
      inoculation of BMCs concurrently with a single dose of antilymphocyte serum 
      induces donor-specific unresponsiveness to rat cardiac allografts. The 
      unresponsiveness may be the result of deletion or functional inactivation of 
      alloreactive clones maturing in a thymus bearing donor alloantigen. Intrathymic 
      inoculation of BMCs deserves further evaluation as a possible clinical strategy 
      for the induction of transplantation tolerance.
FAU - Odorico, J S
AU  - Odorico JS
AD  - Department of Surgery, Hospital of the University of Pennsylvania, Philadelphia 
      19104.
FAU - Barker, C F
AU  - Barker CF
FAU - Posselt, A M
AU  - Posselt AM
FAU - Naji, A
AU  - Naji A
LA  - eng
GR  - DK34878/DK/NIDDK NIH HHS/United States
GR  - DK44309/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Surgery
JT  - Surgery
JID - 0417347
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells
MH  - *Bone Marrow Transplantation
MH  - Graft Survival
MH  - *Heart Transplantation
MH  - *Immune Tolerance
MH  - Injections
MH  - Male
MH  - Rats
MH  - Rats, Inbred Lew
MH  - Rats, Inbred WF
MH  - *Thymus Gland/cytology
MH  - *Tissue Donors
MH  - Transplantation, Homologous
EDAT- 1992/08/01 00:00
MHDA- 1992/08/01 00:01
CRDT- 1992/08/01 00:00
PHST- 1992/08/01 00:00 [pubmed]
PHST- 1992/08/01 00:01 [medline]
PHST- 1992/08/01 00:00 [entrez]
AID - 0039-6060(92)90233-P [pii]
PST - ppublish
SO  - Surgery. 1992 Aug;112(2):370-6; discussion 376-7.