PMID- 16419398
OWN - NLM
STAT- MEDLINE
DCOM- 20060203
LR  - 20191210
IS  - 0146-4760 (Print)
IS  - 0146-4760 (Linking)
VI  - 29
IP  - 7
DP  - 2005 Oct
TI  - Rapid simultaneous determination of amphetamine, methamphetamine, 
      3,4-methylenedioxyamphetamine, 3,4-methylenedioxymethamphetamine, and 
      3,4-methylenedioxyethylamphetamine in urine by fast gas chromatography-mass 
      spectrometry.
PG  - 669-74
AB  - The use of fast gas chromatography-mass spectrometry (FGC-MS) was investigated to 
      improve the efficiency of analysis of urine specimens that previously screened 
      presumptively positive for amphetamine (AMP), methamphetamine (MAMP), 
      3,4-methylenedioxyamphetamine (MDA), 3,4-methylenedioxymethamphetamine (MDMA), 
      and/or 3,4 methylenedioxyethylamphetamine (MDEA) by immunoassay testing. 
      Specimens were pretreated with basic sodium periodate, extracted using a 
      positive-pressure manifold/cation-exchange solid-phase cartridge methodology, and 
      derivatized using 4-carbethoxyhexafluorobutyryl chloride (4-CB). The analytical 
      method was compared to traditional GC-MS analysis and evaluated with respect to 
      assay chromatography, linearity, sensitivity, precision, accuracy, and 
      reproducibility. The limits of detection were 62.5 ng/mL for MDA and 31.25 ng/mL 
      for AMP, MAMP, MDMA, and MDEA. All of the target analytes were linear to 12,000 
      ng/mL with the exception of MAMP which was linear to 10,000 ng/mL. The 
      intra-assay precision of a 500 ng/mL multiconstituent control (n=15) ranged from 
      522.6 to 575.9 ng/mL with a coefficient of variation of less than 3.8%. Authentic 
      human urine specimens (n=187) previously determined to contain the target 
      analytes were re-extracted and analyzed by both FGC-MS and the currently utilized 
      GC-MS method. No significant differences in specimen concentration were observed 
      between these analytical methods. No interferences were seen when the performance 
      of the FGC-MS method was challenged with ephedrine, pseudoephedrine, 
      phenylpropanolamine, and phentermine. When compared to traditional GC-MS 
      analysis, FGC-MS analysis provided a dramatic reduction in retention time for 
      amphetamine (1.8 min vs. 4.12 min). For example, the FGC-MS method reduced 
      overall run time for a batch of 56 specimens from 12.0 h to 7.25 h. This 
      reduction in analysis time makes FGC-MS an attractive alternative to traditional 
      GC-MS by allowing a laboratory greater flexibility in the purchase and use of 
      capital equipment and in the assignment of laboratory personnel, all resulting in 
      greater overall efficiency by decreasing reporting times for AMP, MAMP, and 
      designer amphetamine positive specimens.
FAU - Klette, Kevin L
AU  - Klette KL
AD  - Navy Drug Screening Laboratory, 320B B Street, Great Lakes, Illinois 60088-2815, 
      USA. klklette@nhgl.med.navy.mil
FAU - Jamerson, Matthew H
AU  - Jamerson MH
FAU - Morris-Kukoski, Cynthia L
AU  - Morris-Kukoski CL
FAU - Kettle, Aaron R
AU  - Kettle AR
FAU - Snyder, J Jacob
AU  - Snyder JJ
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PL  - England
TA  - J Anal Toxicol
JT  - Journal of analytical toxicology
JID - 7705085
RN  - 0 (Amphetamines)
RN  - 0 (Central Nervous System Stimulants)
SB  - IM
MH  - Amphetamine-Related Disorders/diagnosis/*urine
MH  - Amphetamines/*urine
MH  - Central Nervous System Stimulants/*urine
MH  - Forensic Medicine/methods
MH  - Gas Chromatography-Mass Spectrometry/*methods
MH  - Humans
MH  - Reproducibility of Results
MH  - Substance Abuse Detection/*methods
EDAT- 2006/01/20 09:00
MHDA- 2006/02/04 09:00
CRDT- 2006/01/20 09:00
PHST- 2006/01/20 09:00 [pubmed]
PHST- 2006/02/04 09:00 [medline]
PHST- 2006/01/20 09:00 [entrez]
AID - 10.1093/jat/29.7.669 [doi]
PST - ppublish
SO  - J Anal Toxicol. 2005 Oct;29(7):669-74. doi: 10.1093/jat/29.7.669.