PMID- 16420445
OWN - NLM
STAT- MEDLINE
DCOM- 20060425
LR  - 20170512
IS  - 0953-816X (Print)
IS  - 0953-816X (Linking)
VI  - 23
IP  - 2
DP  - 2006 Jan
TI  - Localization and function of pre- and postsynaptic kainate receptors in the rat 
      globus pallidus.
PG  - 374-86
AB  - Kainate receptors (KARs) are widely expressed the basal ganglia. In this study, 
      we used electron microscopic immunocytochemistry and whole-cell recording 
      techniques to examine the localization and function of KARs in the rat globus 
      pallidus (GP). Dendrites were the most common immunoreactive elements, while 
      terminals forming symmetric or asymmetric synapses and unmyelinated axons 
      comprised most of the presynaptic labeling. To determine whether synaptically 
      released glutamate activates KARs, we recorded excitatory postsynaptic currents 
      (EPSCs) in the GP following single-pulse stimulation of the internal capsule. 
      4-(8-Methyl-9H-1,3-dioxolo[4,5 h]2,3benzodiazepine-5-yl)-benzenamine 
      hydrochloride (GYKI 52466, 100 microm), an 
      alpha-amino-3-hydroxyl-5-methyl-4-isoxazole propionic acid (AMPA) receptor 
      antagonist, reduced but did not completely block evoked EPSCs. The remaining EPSC 
      component was mediated through activation of KARs because it was abolished by 
      6-cyano-7-nitroquinoxaline-2, 3-dione (CNQX), an AMPA/KAR antagonist. The rise 
      time (10-90%) and decay time constant (tau) for those EPSCs were longer than 
      those of AMPA-mediated EPSCs recorded before GYKI 52466 application. KAR 
      activation inhibited EPSCs. This inhibition was associated with a significant 
      increase in paired-pulse facilitation ratio, suggesting a presynaptic action of 
      KAR. KAR inhibition of EPSCs was blocked by the G-protein inhibitor, 
      N-ethylmaleimide (NEM), or the protein kinase C (PKC) inhibitor calphostin C. Our 
      results demonstrate that KAR activation has dual effects on glutamatergic 
      transmission in the rat GP: (1) it mediates small-amplitude EPSCs; and (2) it 
      reduces glutamatergic synaptic transmission through a presynaptic G-protein 
      coupled, PKC-dependent, metabotropic mechanism. These findings provide evidence 
      for the multifarious functions of KARs in regulating synaptic transmission, and 
      open up the possibility for the development of pharmacotherapies to reduce the 
      hyperactive subthalamofugal projection in Parkinson's disease.
FAU - Jin, Xiao-Tao
AU  - Jin XT
AD  - Division of Neuroscience, Yerkes National Primate Research Center and Department 
      of Neurology, Emory University, 954 Gatewood Road NE, Atlanta, GA 30322, USA.
FAU - Pare, Jean-Francois
AU  - Pare JF
FAU - Raju, Dinesh V
AU  - Raju DV
FAU - Smith, Yoland
AU  - Smith Y
LA  - eng
GR  - T32 GM008169/GM/NIGMS NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - France
TA  - Eur J Neurosci
JT  - The European journal of neuroscience
JID - 8918110
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Excitatory Amino Acid Agonists)
RN  - 0 (Excitatory Amino Acid Antagonists)
RN  - 0 (Receptors, Kainic Acid)
RN  - G6D6147J22 (biocytin)
RN  - K3Z4F929H6 (Lysine)
SB  - IM
MH  - Age Factors
MH  - Animals
MH  - Animals, Newborn
MH  - Cell Count
MH  - Dendrites/drug effects/*physiology
MH  - Dose-Response Relationship, Drug
MH  - Drug Interactions
MH  - Electric Stimulation/methods
MH  - Enzyme Inhibitors/pharmacology
MH  - Excitatory Amino Acid Agonists/pharmacology
MH  - Excitatory Amino Acid Antagonists/pharmacology
MH  - Excitatory Postsynaptic Potentials/drug effects/physiology/radiation effects
MH  - Globus Pallidus/*cytology/growth & development
MH  - In Vitro Techniques
MH  - Lysine/analogs & derivatives/metabolism
MH  - Microscopy, Immunoelectron/methods
MH  - Neurons/*physiology
MH  - Patch-Clamp Techniques/methods
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, Kainic Acid/*metabolism
MH  - Synapses/*physiology
MH  - Synaptic Transmission/drug effects/*physiology/radiation effects
EDAT- 2006/01/20 09:00
MHDA- 2006/04/28 09:00
CRDT- 2006/01/20 09:00
PHST- 2006/01/20 09:00 [pubmed]
PHST- 2006/04/28 09:00 [medline]
PHST- 2006/01/20 09:00 [entrez]
AID - EJN4574 [pii]
AID - 10.1111/j.1460-9568.2005.04574.x [doi]
PST - ppublish
SO  - Eur J Neurosci. 2006 Jan;23(2):374-86. doi: 10.1111/j.1460-9568.2005.04574.x.