PMID- 16420624
OWN - NLM
STAT- MEDLINE
DCOM- 20060417
LR  - 20061115
IS  - 0168-6496 (Print)
IS  - 0168-6496 (Linking)
VI  - 55
IP  - 1
DP  - 2006 Jan
TI  - A digital imaging procedure for seven-probe-labeling FISH (Rainbow-FISH) and its 
      application to estuarine microbial communities.
PG  - 159-66
AB  - For multi-probe-labeling fluorescence in situ hybridization (FISH), a digital 
      imaging procedure was developed consisting of systematic background noise 
      reduction and target signal equalization using a hue, saturation, value color 
      partitioning technique. By the combined application of seven DNA probes, each 
      labeled with three fluorochromes at maximum, seven kinds of cultured type strains 
      were distinguished in a microscopic field simultaneously. Using this 
      seven-probe-labeling FISH (Rainbow-FISH), several phylogenetic groups of microbes 
      that occur frequently in aquatic environments, such as Alpha-, Beta- and 
      Gammaproteobacteria, Cytophaga-Flavobacterium and Actinobacteria, were identified 
      and quantified. The total counts of cells specified by Rainbow-FISH were in the 
      range of 96-108% of those of general FISH, showing that the method is highly 
      reliable for quantitative population analysis. Analyzing samples obtained at 
      points along a river to a sea, we found a reverse population change in two 
      groups: apparent decreases in Betaproteobacteria but gradual increases in 
      Gammaproteobacteria. This method provides a platform toward the improvement of 
      semiautomatic analysis of aquatic microbes under various metabolic conditions.
FAU - Sunamura, Michinari
AU  - Sunamura M
AD  - Biological Resources and Functions, National Institute of Advanced and Industrial 
      Science and Technology, AIST, Tsukuba, Ibaraki, Japan.
FAU - Maruyama, Akihiko
AU  - Maruyama A
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - FEMS Microbiol Ecol
JT  - FEMS microbiology ecology
JID - 8901229
RN  - 0 (Oligonucleotide Probes)
SB  - IM
MH  - Actinobacteria/genetics/isolation & purification
MH  - Archaea/genetics
MH  - Colony Count, Microbial/methods
MH  - Computers
MH  - Cytophaga/genetics/isolation & purification
MH  - Fresh Water/microbiology
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Japan
MH  - Marine Biology/*methods
MH  - Oligonucleotide Probes
MH  - Proteobacteria/genetics/isolation & purification
MH  - *Water Microbiology
EDAT- 2006/01/20 09:00
MHDA- 2006/04/18 09:00
CRDT- 2006/01/20 09:00
PHST- 2006/01/20 09:00 [pubmed]
PHST- 2006/04/18 09:00 [medline]
PHST- 2006/01/20 09:00 [entrez]
AID - FEM013 [pii]
AID - 10.1111/j.1574-6941.2005.00013.x [doi]
PST - ppublish
SO  - FEMS Microbiol Ecol. 2006 Jan;55(1):159-66. doi: 
      10.1111/j.1574-6941.2005.00013.x.