PMID- 16423045
OWN - NLM
STAT- MEDLINE
DCOM- 20060206
LR  - 20181113
IS  - 0019-2805 (Print)
IS  - 1365-2567 (Electronic)
IS  - 0019-2805 (Linking)
VI  - 117
IP  - 1
DP  - 2006 Jan
TI  - The delivery of an antigen from the endocytic compartment into the cytosol for 
      cross-presentation is restricted to early immature dendritic cells.
PG  - 97-107
AB  - Dendritic cells (DCs) are the only antigen-presenting cell population having a 
      cross-presentation capacity. For cross-presentation, however, the intracellular 
      antigen-processing pathway and its regulatory mechanism have not been defined. 
      Here we report the differences in cross-presentation ability among murine bone 
      marrow-derived immature DC, early immature day8-DC and late immature day10-DC, 
      and fully mature day10 + lipopolysaccharide DC. Day8-DCs and day10-DCs show an 
      immature phenotypic profile but are different in morphology. Day8-DCs can 
      internalize an abundant volume of exogenous soluble ovalbumin (OVA) and result in 
      cross-presentation. In contrast, day10-DCs are not able to cross-present, 
      although they maintain efficient macropinocytosis. Exogenously internalized OVA 
      antigens are stored in the endocytic compartments. The endocytic compartments are 
      temporarily maintained at mildly acidic pH in day8-DCs and are rapidly acidified 
      in day10-DCs after uptake of antigens. We show that OVA antigens accumulated in 
      the endocytic compartments move into the cytosol in day8-DCs but do not in 
      day10-DCs. NH(4)Cl-treatment, which neutralizes the acidic endocytic compartments 
      and/or delays endosomal maturation, restores day10-DCs for transport the stored 
      OVA antigens from the endocytic compartments into the cytosol. 
      Diphenyleneiodonium chloride-treatment, which acidifies the endocytic 
      compartments, decreases an amount of transported OVA antigen into the cytosol in 
      day8-DCs. These data indicate that only the early immature stage of DC interferes 
      with endosomal maturation, even after uptake of exogenous antigens, and then 
      transports the antigens into the cytosol.
FAU - Hotta, Chie
AU  - Hotta C
AD  - Department of Immunology, Yokohama City University School of Medicine, Yokohama, 
      Japan.
FAU - Fujimaki, Haruka
AU  - Fujimaki H
FAU - Yoshinari, Masahiro
AU  - Yoshinari M
FAU - Nakazawa, Masatoshi
AU  - Nakazawa M
FAU - Minami, Mutsuhiko
AU  - Minami M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Immunology
JT  - Immunology
JID - 0374672
RN  - 0 (Antigens)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 01Q9PC255D (Ammonium Chloride)
RN  - 9006-59-1 (Ovalbumin)
SB  - IM
MH  - Ammonium Chloride/pharmacology
MH  - Animals
MH  - *Antigen Presentation
MH  - Antigens/immunology/metabolism
MH  - Biological Transport/drug effects/immunology
MH  - Cell Differentiation/immunology
MH  - Cross-Priming/drug effects/*immunology
MH  - Cytosol/immunology
MH  - Dendritic Cells/drug effects/*immunology
MH  - Histocompatibility Antigens Class I/immunology
MH  - Hydrogen-Ion Concentration
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Ovalbumin/immunology/pharmacokinetics
MH  - Transport Vesicles/immunology
PMC - PMC1782205
EDAT- 2006/01/21 09:00
MHDA- 2006/02/07 09:00
PMCR- 2007/01/01
CRDT- 2006/01/21 09:00
PHST- 2006/01/21 09:00 [pubmed]
PHST- 2006/02/07 09:00 [medline]
PHST- 2006/01/21 09:00 [entrez]
PHST- 2007/01/01 00:00 [pmc-release]
AID - IMM2270 [pii]
AID - 10.1111/j.1365-2567.2005.02270.x [doi]
PST - ppublish
SO  - Immunology. 2006 Jan;117(1):97-107. doi: 10.1111/j.1365-2567.2005.02270.x.