PMID- 16423659
OWN - NLM
STAT- MEDLINE
DCOM- 20060328
LR  - 20061115
IS  - 0723-2020 (Print)
IS  - 0723-2020 (Linking)
VI  - 29
IP  - 1
DP  - 2006 Jan
TI  - PCR-based DGGE fingerprinting and identification of methanogens detected in three 
      different types of UASB granules.
PG  - 77-84
AB  - Methane is produced by various methanogenic bacteria present in upflow anaerobic 
      sludge blanket (UASB) bioreactors. Methane can be used to predict and improve 
      UASB bioreactor efficiency. The methanogen population in the granules can be 
      influenced by the composition of the substrate. The aim of this study was to 
      fingerprint and identify the methanogens present in three different types of UASB 
      granules that had been used to treat winery, brewery and peach-lye canning 
      effluents. This was done using polymerase chain reaction (PCR)-based denaturing 
      gradient gel electrophoresis (DGGE) and DNA sequence analysis. The DGGE 
      fingerprints obtained from the methanogen reference cultures of Methanosaeta 
      concilii, Methanosaeta thermophila, Methanosarcina barkeri, Methanosarcina mazeii 
      and Methanobacterium formicicum were compared to the DGGE profiles of the Archaea 
      in the different granules. The positions of the DGGE bands that did not 
      correspond well to the bands of the known species were sequenced and compared to 
      sequences available on GenBank using the Blastn search option. The aligned DNA 
      sequences were used to construct a phylogenetic tree. Based on the data obtained, 
      a DGGE marker was constructed which was used to provide a quick method to 
      identify the Archaeal members of the microbial consortium in UASB granules.
FAU - Keyser, M
AU  - Keyser M
AD  - Department of Food Science, Stellenbosch University, Private Bag X1, Matieland 
      7602, South Africa.
FAU - Witthuhn, R C
AU  - Witthuhn RC
FAU - Lamprecht, C
AU  - Lamprecht C
FAU - Coetzee, M P A
AU  - Coetzee MP
FAU - Britz, T J
AU  - Britz TJ
LA  - eng
SI  - GENBANK/AY904330
SI  - GENBANK/AY904331
SI  - GENBANK/AY904332
SI  - GENBANK/AY904333
SI  - GENBANK/AY904334
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20050726
PL  - Germany
TA  - Syst Appl Microbiol
JT  - Systematic and applied microbiology
JID - 8306133
RN  - 0 (DNA, Archaeal)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Sewage)
SB  - IM
MH  - Base Sequence
MH  - Bioreactors/microbiology
MH  - DNA Fingerprinting
MH  - DNA, Archaeal/chemistry/genetics
MH  - Electrophoresis/methods
MH  - Methanobacteriaceae/*classification/genetics/growth & development
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - Polymerase Chain Reaction/*methods
MH  - RNA, Ribosomal, 16S/chemistry/genetics
MH  - Sequence Alignment
MH  - Sewage/microbiology
EDAT- 2006/01/21 09:00
MHDA- 2006/03/29 09:00
CRDT- 2006/01/21 09:00
PHST- 2005/05/27 00:00 [received]
PHST- 2006/01/21 09:00 [pubmed]
PHST- 2006/03/29 09:00 [medline]
PHST- 2006/01/21 09:00 [entrez]
AID - S0723-2020(05)00127-X [pii]
AID - 10.1016/j.syapm.2005.06.003 [doi]
PST - ppublish
SO  - Syst Appl Microbiol. 2006 Jan;29(1):77-84. doi: 10.1016/j.syapm.2005.06.003. Epub 
      2005 Jul 26.