PMID- 16424349
OWN - NLM
STAT- MEDLINE
DCOM- 20060714
LR  - 20141120
IS  - 1524-4636 (Electronic)
IS  - 1079-5642 (Linking)
VI  - 26
IP  - 4
DP  - 2006 Apr
TI  - Influence of folate on arterial permeability and stiffness in the absence or 
      presence of hyperhomocysteinemia.
PG  - 814-8
AB  - OBJECTIVE: Elevated plasma total homocysteine (tHcy) is associated with risk for 
      cardiovascular disease. A common cause of mild hyperhomocysteinemia (HHcy) is 
      folate deficiency. We sought to determine whether folate deficiency per se 
      increases arterial permeability (quantitative fluorescence microscopy) and 
      stiffness (vessel elastigraph), and whether the effects of folate deficiency are 
      more severe in the presence of mild HHcy. METHODS AND RESULTS: Heterozygous 
      cystathionine beta-synthase (CBS)-deficient mice (CBS(+/-)) and their wild-type 
      littermates (CBS(+/+)) were fed chow containing either standard (Con) or 
      relatively low amounts of folate (LF) for 18+/-3 weeks. Liver folate (microg 
      folate/g liver) and tHcy (microM), respectively, were 12+/-1 and 8+/-1 in 
      CBS(+/+) Con mice (n=12), and 8+/-1 and 8+/-1 in CBS(+/+) LF animals (n=5). 
      Carotid arterial permeability was &38% greater (P<0.05) in CBS(+/+) LF versus Con 
      mice, but vascular stiffening was unaltered. Liver folate and tHcy, respectively, 
      were 13+/-1 and 11+/-1 in CBS(+/-) Con mice (n=16), and 8+/-1 and 16+/-3 in 
      CBS(+/-) LF animals (n=6). Carotid arterial dextran accumulation was &31% 
      greater, and maximal strain in aortae was &20% lower (both P<0.05) in CBS(+/-) LF 
      versus Con mice. CONCLUSIONS: Taken together, low folate (P<0.05) combined with 
      mild HHcy (P<0.05) in CBS(+/-) mice produced more arterial dysfunction compared 
      with low folate alone (ie, CBS(+/+) mice). These findings may be particularly 
      relevant to elderly individuals because tHcy and deficiencies of folate 
      metabolism increase with age.
FAU - Symons, J David
AU  - Symons JD
AD  - College of Health, University of Utah, Salt Lake City, Utah, USA. 
      j.david.symons@hsc.utah.edu
FAU - Zaid, Ussama B
AU  - Zaid UB
FAU - Athanassious, Christian N
AU  - Athanassious CN
FAU - Mullick, Adam E
AU  - Mullick AE
FAU - Lentz, Steven R
AU  - Lentz SR
FAU - Rutledge, John C
AU  - Rutledge JC
LA  - eng
GR  - 35747/PHS HHS/United States
GR  - HL55667/HL/NHLBI NIH HHS/United States
GR  - HL63943/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20060119
PL  - United States
TA  - Arterioscler Thromb Vasc Biol
JT  - Arteriosclerosis, thrombosis, and vascular biology
JID - 9505803
RN  - 935E97BOY8 (Folic Acid)
RN  - EC 4.2.1.22 (Cystathionine beta-Synthase)
SB  - IM
MH  - Animals
MH  - Capillary Permeability/*drug effects
MH  - Carotid Arteries/drug effects/*metabolism/physiopathology
MH  - Cystathionine beta-Synthase/*biosynthesis/deficiency
MH  - Endothelium, Vascular/metabolism/physiopathology
MH  - Folic Acid/*administration & dosage/blood/pharmacokinetics
MH  - Humans
MH  - Hyperhomocysteinemia/blood/drug therapy/physiopathology
MH  - In Vitro Techniques
MH  - Mice
MH  - Vasoconstriction/drug effects
EDAT- 2006/01/21 09:00
MHDA- 2006/07/15 09:00
CRDT- 2006/01/21 09:00
PHST- 2006/01/21 09:00 [pubmed]
PHST- 2006/07/15 09:00 [medline]
PHST- 2006/01/21 09:00 [entrez]
AID - 01.ATV.0000204408.01416.16 [pii]
AID - 10.1161/01.ATV.0000204408.01416.16 [doi]
PST - ppublish
SO  - Arterioscler Thromb Vasc Biol. 2006 Apr;26(4):814-8. doi: 
      10.1161/01.ATV.0000204408.01416.16. Epub 2006 Jan 19.