PMID- 16424399
OWN - NLM
STAT- MEDLINE
DCOM- 20070118
LR  - 20220331
IS  - 1066-5099 (Print)
IS  - 1066-5099 (Linking)
VI  - 24
IP  - 5
DP  - 2006 May
TI  - Selenium supplementation restores the antioxidative capacity and prevents cell 
      damage in bone marrow stromal cells in vitro.
PG  - 1226-35
AB  - Bone marrow stromal cells (BMSCs) and other cell populations derived from 
      mesenchymal precursors are developed for cell-based therapeutic strategies and 
      undergo cellular stress during ex vivo procedures. Reactive oxygen species (ROS) 
      of cellular and environmental origin are involved in redox signaling, cumulative 
      cell damage, senescence, and tumor development. Selenium-dependent (glutathione 
      peroxidases [GPxs] and thioredoxin reductases [TrxRs]) and selenium-independent 
      (superoxide dismutases [SODs] and catalase [CAT]) enzyme systems regulate 
      cellular ROS steady state levels. SODs process superoxide anion to hydrogen 
      peroxide, which is subsequently neutralized by GPx and CAT; TrxR neutralizes 
      other ROS, such as peroxinitrite. Primary BMSCs and telomerase-immortalized human 
      mesenchymal stem cells (hMSC-TERT) express GPx1-3, TrxR1, TrxR2, SOD1, SOD2, and 
      CAT. We show here that in standard cell cultures (5%-10% fetal calf serum, 5-10 
      nM selenite), the activity of antioxidative selenoenzymes is impaired in 
      hMSC-TERT and BMSCs. Under these conditions, the superoxide anion processing 
      enzyme SOD1 is not sufficiently stimulated by an ROS load. Resulting oxidative 
      stress favors generation of micronuclei in BMSCs. Supplementation of selenite 
      (100 nM) restores basal GPx and TrxR activity, rescues basal and ROS-stimulated 
      SOD1 mRNA expression and activity, and reduces ROS accumulation in hMSC-TERT and 
      micronuclei generation in BMSCs. In conclusion, BMSCs in routine cell culture 
      have low antioxidative capacity and are subjected to oxidative stress, as 
      indicated by the generation of micronuclei. Selenite supplementation of BMSC 
      cultures appears to be an important countermeasure to restore their antioxidative 
      capacity and to reduce cell damage in the context of tissue engineering and 
      transplantation procedures.
FAU - Ebert, Regina
AU  - Ebert R
AD  - Musculosceletal Research Center, Orthopaedic Department, University of Wurzburg, 
      Brettreichstrasse 11, D-97074 Wurzburg, Germany.
FAU - Ulmer, Matthias
AU  - Ulmer M
FAU - Zeck, Sabine
AU  - Zeck S
FAU - Meissner-Weigl, Jutta
AU  - Meissner-Weigl J
FAU - Schneider, Doris
AU  - Schneider D
FAU - Stopper, Helga
AU  - Stopper H
FAU - Schupp, Nicole
AU  - Schupp N
FAU - Kassem, Moustapha
AU  - Kassem M
FAU - Jakob, Franz
AU  - Jakob F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060119
PL  - England
TA  - Stem Cells
JT  - Stem cells (Dayton, Ohio)
JID - 9304532
RN  - 0 (Culture Media)
RN  - 0 (Reactive Oxygen Species)
RN  - 63231-63-0 (RNA)
RN  - EC 1.- (Oxidoreductases)
RN  - H6241UJ22B (Selenium)
SB  - IM
MH  - Base Sequence
MH  - Bone Marrow Cells/*drug effects/enzymology
MH  - Cell Proliferation/drug effects
MH  - Cells, Cultured
MH  - Culture Media/chemistry
MH  - *DNA Damage
MH  - Humans
MH  - Mesenchymal Stem Cells/drug effects/enzymology
MH  - Micronucleus Tests
MH  - Molecular Sequence Data
MH  - Oxidoreductases/drug effects/genetics/metabolism
MH  - RNA
MH  - Reactive Oxygen Species/antagonists & inhibitors/*metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Selenium/*pharmacology
MH  - Stromal Cells/*drug effects/enzymology
EDAT- 2006/01/21 09:00
MHDA- 2007/01/19 09:00
CRDT- 2006/01/21 09:00
PHST- 2006/01/21 09:00 [pubmed]
PHST- 2007/01/19 09:00 [medline]
PHST- 2006/01/21 09:00 [entrez]
AID - 2005-0117 [pii]
AID - 10.1634/stemcells.2005-0117 [doi]
PST - ppublish
SO  - Stem Cells. 2006 May;24(5):1226-35. doi: 10.1634/stemcells.2005-0117. Epub 2006 
      Jan 19.