PMID- 16425417
OWN - NLM
STAT- MEDLINE
DCOM- 20060321
LR  - 20190430
IS  - 1007-9327 (Print)
IS  - 2219-2840 (Electronic)
IS  - 1007-9327 (Linking)
VI  - 11
IP  - 41
DP  - 2005 Nov 7
TI  - Bile salts inhibit growth and induce apoptosis of culture human normal esophageal 
      mucosal epithelial cells.
PG  - 6466-71
AB  - AIM: To investigate the effect of six bile salts: glycocholate (GC), 
      glycochenodeoxycholate (GCDC), glycodeoxycholate (GDC), taurocholate (TC), 
      taurochenodeoxycholate (TCDC), taurodeoxycholate (TDC), and their mixture on 
      cultured human normal esophageal mucosal epithelial cells. METHODS: Human normal 
      esophageal mucosal epithelial cells were cultured with serum-free keratinocyte 
      medium.3-[4,5-Dimethylthiaolyl]-2,5-diphenyl-tetrazolium bromide assay was 
      applied to the detection of cell proliferation. Apoptotic morphology was observed 
      by phase-contrast video microscopy and terminal deoxynucleotidyl 
      transferase-mediated dUTP nick end labeling (TUNEL) assay. Sub-G1 DNA 
      fragmentations and early apoptotic cells were assayed by flow cytometry (FCM) 
      with propidium iodide (PI) staining and annexin V-FITC conjugated with PI 
      staining. Apoptotic DNA ladders on agarose gel electrophoresis were observed. 
      RESULTS: Except for GC, GCDC, GDC, TC, TCDC, TDC and their mixture could initiate 
      growth inhibition of esophageal mucosal epithelial cells in a dose- and 
      time-dependent manner. TUNEL and FCM assays demonstrated that the bile salts at 
      500 mumol/L and their mixture at 1 500 micromol/L induced apoptosis except for 
      GC. The percentage of sub-G1 detected by FCM with PI staining was 83.5% in cells 
      treated with 500 micromol/L TC for 2 h, and 19.8%, 20.4%, 25.6%, 13.5%, and 75.8% 
      in cells treated with 500 micromol/L GCDC, TCDC, GDC, TDC, and 1 500 micromol/L 
      mixture for 24 h, respectively, which were higher than that of the control 
      (1.5%). The percentage was 1.4% in cells with 500 micromol/L GC for 24 h. DNA 
      ladders on agarose gel electrophoresis were seen in cells treated with 500 
      micromol/L TC for 2 h and 1 500 micromol/L mixture for 24 h. CONCLUSION: All 
      GCDC, GDC, TC, TCDC, TDC and their mixture can inhibit growth and induce 
      apoptosis of cultured human normal esophageal mucosal epithelial cells, but GC is 
      well tolerated by the cells.
FAU - Zhang, Ru
AU  - Zhang R
AD  - Digestive Department of the Second Hospital, Xioan Jiaotong University, Xioan 
      710004, Shaanxi Province, China.
FAU - Gong, Jun
AU  - Gong J
FAU - Wang, Hui
AU  - Wang H
FAU - Wang, Li
AU  - Wang L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - World J Gastroenterol
JT  - World journal of gastroenterology
JID - 100883448
RN  - 0 (Bile Acids and Salts)
SB  - IM
MH  - Apoptosis/*drug effects
MH  - Bile Acids and Salts/*pharmacology
MH  - Cell Division/drug effects
MH  - Cells, Cultured
MH  - Epithelial Cells/*cytology/*drug effects
MH  - Esophagus/*cytology
MH  - Humans
MH  - Mucous Membrane/cytology
PMC - PMC4355787
EDAT- 2006/01/21 09:00
MHDA- 2006/03/22 09:00
PMCR- 2005/11/07
CRDT- 2006/01/21 09:00
PHST- 2006/01/21 09:00 [pubmed]
PHST- 2006/03/22 09:00 [medline]
PHST- 2006/01/21 09:00 [entrez]
PHST- 2005/11/07 00:00 [pmc-release]
AID - 10.3748/wjg.v11.i41.6466 [doi]
PST - ppublish
SO  - World J Gastroenterol. 2005 Nov 7;11(41):6466-71. doi: 10.3748/wjg.v11.i41.6466.