PMID- 16426682
OWN - NLM
STAT- MEDLINE
DCOM- 20060522
LR  - 20131121
IS  - 0161-813X (Print)
IS  - 0161-813X (Linking)
VI  - 27
IP  - 2
DP  - 2006 Mar
TI  - Reactive species and apoptosis of neural precursor cells after gamma-irradiation.
PG  - 253-9
AB  - This study addresses the participation of radiation-induced free radicals, mainly 
      nitric oxide (NO), in modulating the apoptotic response in an in vitro model of 
      neural cortical precursor cells exposed to gamma-radiation. Cortical cells 
      obtained from rats at 17 gestational day (GD) were irradiated with a dose of 2 
      Gy. The percentage of apoptotic cells was significantly increased 4h 
      post-irradiation (pi). NO content showed a significant increase after 30 min pi 
      and the rate of generation reached a maximum 1h pi. Luminol-dependent 
      chemiluminescence (CL) was significantly higher in cells after 2h pi as compared 
      to control cells and this profile was maintained up to 4 h pi. Supplementation 
      with L-NAME significantly increased light emission. Administration of superoxide 
      dismutase (SOD) following L-NAME addition prevented the observed changes due to 
      L-NAME administration. The caspase inhibitor zDEVD-fmk significantly reduced the 
      radical generation. Moreover, the cellular decrease in NO content occurred 
      coincidentally with the rise in oxygen radical generation and the activation of 
      caspase-3. In vitro irradiation of neural precursor cells allowed us to suggest 
      that an early radiation-induced generation of NO could exert a neuroprotective 
      role. However, despite this NO initial protective effect and its role modulating 
      the response against gamma-radiation, NO generation was not able of fully 
      preventing radiation-induced apoptosis.
FAU - Gisone, Pablo
AU  - Gisone P
AD  - Nuclear Regulatory Authority, Radiopathology Laboratory, Avenida del Libertador 
      8250 (C1429BNP) Buenos Aires, Argentina.
FAU - Robello, Elizabeth
AU  - Robello E
FAU - Sanjurjo, Julieta
AU  - Sanjurjo J
FAU - Dubner, Diana
AU  - Dubner D
FAU - Perez, Maria del Rosario
AU  - Perez Mdel R
FAU - Michelin, Severino
AU  - Michelin S
FAU - Puntarulo, Susana
AU  - Puntarulo S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060119
PL  - Netherlands
TA  - Neurotoxicology
JT  - Neurotoxicology
JID - 7905589
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Nitrates)
RN  - 0 (Nitrites)
RN  - 0 (Reactive Oxygen Species)
RN  - 11062-77-4 (Superoxides)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type I)
RN  - EC 3.4.22.- (Casp3 protein, rat)
RN  - EC 3.4.22.- (Caspase 3)
RN  - EC 3.4.22.- (Caspases)
RN  - V55S2QJN2X (NG-Nitroarginine Methyl Ester)
SB  - IM
MH  - Animals
MH  - Apoptosis/*radiation effects
MH  - Caspase 3
MH  - Caspases/metabolism
MH  - Cells, Cultured
MH  - Enzyme Inhibitors/pharmacology
MH  - Flow Cytometry
MH  - Gamma Rays
MH  - Luminescent Measurements
MH  - NG-Nitroarginine Methyl Ester/pharmacology
MH  - Neurons/*radiation effects
MH  - Nitrates/metabolism
MH  - Nitric Oxide/metabolism
MH  - Nitric Oxide Synthase Type I/antagonists & inhibitors
MH  - Nitrites/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Reactive Oxygen Species/*metabolism
MH  - Stem Cells/*radiation effects
MH  - Superoxides/metabolism
EDAT- 2006/01/24 09:00
MHDA- 2006/05/23 09:00
CRDT- 2006/01/24 09:00
PHST- 2005/08/19 00:00 [received]
PHST- 2005/11/14 00:00 [revised]
PHST- 2005/11/14 00:00 [accepted]
PHST- 2006/01/24 09:00 [pubmed]
PHST- 2006/05/23 09:00 [medline]
PHST- 2006/01/24 09:00 [entrez]
AID - S0161-813X(05)00210-X [pii]
AID - 10.1016/j.neuro.2005.11.006 [doi]
PST - ppublish
SO  - Neurotoxicology. 2006 Mar;27(2):253-9. doi: 10.1016/j.neuro.2005.11.006. Epub 
      2006 Jan 19.