PMID- 16426684
OWN - NLM
STAT- MEDLINE
DCOM- 20060803
LR  - 20061115
IS  - 0166-0934 (Print)
IS  - 0166-0934 (Linking)
VI  - 134
IP  - 1-2
DP  - 2006 Jun
TI  - Peptide ELISA for measuring antibodies to N-protein of porcine reproductive and 
      respiratory syndrome virus.
PG  - 99-118
AB  - Indirect and competition ELISAs with synthetic peptides were used to characterize 
      the epitopes of the N-protein of porcine reproductive and respiratory syndrome 
      virus (PRRSV) that are recognized by a battery of monoclonal antibodies (mAbs) 
      and by antibodies from infected pigs. Four linear epitopes recognized by mAbs 
      have been identified in the most hydrophilic segment of the N-protein (AA25-57). 
      Similarly, at least four linear epitopes in this segment are immunogenic in 
      PRRSV-infected pigs, but only one corresponds to an epitope recognized by one of 
      the mAbs (AA36-45). Antibody formation to these epitopes varied greatly between 
      individual pigs. Most infected pigs generated antibodies that bound to both 
      peptides and HerdChek plates, which are commonly used in the sero-diagnosis of 
      PRRSV infections, but the time course of formation of peptide binding antibodies 
      and antibodies that react with HerdChek plates differed greatly between pigs. 
      This suggests that, although the peptide and HerdChek ELISAs may detect 
      antibodies to some of the same epitopes, they also seem to detect antibodies to 
      epitopes that are uniquely expressed by one and not the other. Some mAbs fail to 
      bind to HerdChek ELISA plates and this is also the case for certain pig 
      antibodies. Peptide ELISA results identified four herds in which most or all pigs 
      possessed N-protein peptide binding antibodies, even though they were HerdChek 
      ELISA sero-negative and exhibited no other signs of PRRSV infection. Thus PRRSV 
      infections may be more widespread than presently realized involving strains that 
      cause asymptomatic infections. The peptide ELISA is useful as an adjunct to the 
      HerdChek ELISA or it could replace it since only two serum samples among 450 
      tested were HerdChek ELISA positive but peptide ELISA negative. The peptide ELISA 
      is also considerably cheaper than the HerdChek ELISA, more flexible and can 
      provide information on the epitope specificity of the reacting antibodies.
FAU - Plagemann, Peter G W
AU  - Plagemann PG
AD  - Department of Microbiology, Medical School, University of Minnesota, 1460 Mayo 
      Memorial Building, Box 196, 420 Delaware Street S.E., Minneapolis, MN 55455-0312, 
      USA. plage001@umn.edu
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060119
PL  - Netherlands
TA  - J Virol Methods
JT  - Journal of virological methods
JID - 8005839
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antibodies, Viral)
RN  - 0 (Epitopes)
RN  - 0 (Nucleocapsid Proteins)
RN  - 0 (Peptides)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antibodies, Monoclonal/immunology
MH  - Antibodies, Viral/*blood
MH  - Antibody Specificity
MH  - Enzyme-Linked Immunosorbent Assay/*methods
MH  - Epitopes/genetics/immunology
MH  - Molecular Sequence Data
MH  - Nucleocapsid Proteins/genetics/*immunology
MH  - Peptides/chemical synthesis
MH  - Porcine Reproductive and Respiratory Syndrome/*blood
MH  - Porcine respiratory and reproductive syndrome virus/genetics/*immunology
MH  - Sequence Alignment
MH  - Swine
EDAT- 2006/01/24 09:00
MHDA- 2006/08/04 09:00
CRDT- 2006/01/24 09:00
PHST- 2005/10/03 00:00 [received]
PHST- 2005/12/04 00:00 [revised]
PHST- 2005/12/08 00:00 [accepted]
PHST- 2006/01/24 09:00 [pubmed]
PHST- 2006/08/04 09:00 [medline]
PHST- 2006/01/24 09:00 [entrez]
AID - S0166-0934(05)00416-7 [pii]
AID - 10.1016/j.jviromet.2005.12.003 [doi]
PST - ppublish
SO  - J Virol Methods. 2006 Jun;134(1-2):99-118. doi: 10.1016/j.jviromet.2005.12.003. 
      Epub 2006 Jan 19.