PMID- 16426705
OWN - NLM
STAT- MEDLINE
DCOM- 20060526
LR  - 20061115
IS  - 0197-0186 (Print)
IS  - 0197-0186 (Linking)
VI  - 48
IP  - 4
DP  - 2006 Mar
TI  - Increased glutamine synthetase but normal EAAT2 expression in platelets of ALS 
      patients.
PG  - 306-11
AB  - Amyotrophic lateral sclerosis is a fatal neurodegenerative disease and glutamate 
      excitotoxicity has been implicated in its pathogenesis. Platelets contain a 
      glutamate uptake system and express components of the glutamate-glutamine cycle, 
      such as the predominant glial excitatory amino acid transporter 2 (EAAT2). In 
      several neurological diseases platelets have proven to be systemic markers for 
      the disease. We compared properties of key components of the glutamate-glutamine 
      cycle in blood platelets of ALS patients and healthy controls. Platelets were 
      analyzed for (3)H-glutamate uptake in the presence or absence of thrombin and for 
      EAAT2 and glutamine synthetase protein expression by Western blotting. Platelets 
      of ALS patients showed a 37% increase in expression of glutamine synthetase, but 
      normal expression of glutamate transporter EAAT2. Glutamate uptake in resting or 
      thrombin-stimulated platelets did not differ significantly between platelets from 
      ALS patients and controls. Thrombin-stimulation resulted in about a seven-fold 
      increase in glutamate uptake. Our data suggest that glutamine synthetase may be a 
      peripheral marker of ALS and encourage further investigation into the role of 
      this enzyme in ALS.
FAU - Bos, I W M
AU  - Bos IW
AD  - Rudolf Magnus Institute of Neuroscience, Department of Pharmacology and Anatomy, 
      University Medical Center, Utrecht, The Netherlands.
FAU - Hoogland, G
AU  - Hoogland G
FAU - Meine Jansen, C F
AU  - Meine Jansen CF
FAU - Willigen, G van
AU  - Willigen Gv
FAU - Spierenburg, H A
AU  - Spierenburg HA
FAU - van den Berg, L H
AU  - van den Berg LH
FAU - de Graan, P N E
AU  - de Graan PN
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060119
PL  - England
TA  - Neurochem Int
JT  - Neurochemistry international
JID - 8006959
RN  - 0 (Excitatory Amino Acid Transporter 2)
RN  - EC 6.3.1.2 (Glutamate-Ammonia Ligase)
SB  - IM
MH  - Adult
MH  - Amyotrophic Lateral Sclerosis/blood/enzymology/*metabolism
MH  - Blood Platelets/enzymology/*metabolism
MH  - Blotting, Western
MH  - Case-Control Studies
MH  - Excitatory Amino Acid Transporter 2/*blood
MH  - Female
MH  - Glutamate-Ammonia Ligase/*blood
MH  - Humans
MH  - Male
MH  - Middle Aged
EDAT- 2006/01/24 09:00
MHDA- 2006/05/27 09:00
CRDT- 2006/01/24 09:00
PHST- 2005/07/04 00:00 [received]
PHST- 2005/09/09 00:00 [revised]
PHST- 2005/09/27 00:00 [accepted]
PHST- 2006/01/24 09:00 [pubmed]
PHST- 2006/05/27 09:00 [medline]
PHST- 2006/01/24 09:00 [entrez]
AID - S0197-0186(05)00259-7 [pii]
AID - 10.1016/j.neuint.2005.09.009 [doi]
PST - ppublish
SO  - Neurochem Int. 2006 Mar;48(4):306-11. doi: 10.1016/j.neuint.2005.09.009. Epub 
      2006 Jan 19.