PMID- 16427600
OWN - NLM
STAT- MEDLINE
DCOM- 20060414
LR  - 20131121
IS  - 0003-9861 (Print)
IS  - 0003-9861 (Linking)
VI  - 446
IP  - 2
DP  - 2006 Feb 15
TI  - Cytoplasmic localization and efflux of endogenous D-aspartate in pheochromocytoma 
      12 cells.
PG  - 131-9
AB  - In our previous reports [Z. Long, H. Homma, J.-A. Lee, T. Fukushima, T. Santa, T. 
      Iwatsubo, R. Yamada, K. Imai, FEBS Lett. 434 (1998) 231-235; Z. Long, M. Sekine, 
      M. Adachi, T. Furuchi, K. Imai, N. Nimura, H. Homma, Arch. Biochem. Biophys. 404 
      (2002) 92-97], we demonstrated for the first time that D-aspartate (D-Asp) is 
      actually synthesized in cultured mammalian cells such as PC12, MPT1, and GH3 
      cells. After its synthesis, this unique amino acid is spontaneously and 
      continuously released into the extracellular space during cell culture. In the 
      current study, we characterized two different types of D-Asp efflux in PC12 
      cells. One is a spontaneous and continuous form of release of cytoplasmic origin 
      that does not involve exocytotic efflux of vesicular origin. Endogenous D-Asp is 
      predominantly localized to the cytoplasm of cells, and this form of D-Asp release 
      presents a striking contrast to exocytotic, quantal discharge of vesicular 
      dopamine. The other form of efflux is also of cytoplasmic origin and occurs 
      through volume-sensitive organic anion channels that are opened upon hyposmotic 
      stimuli. Interestingly, this latter form of efflux is potentiated by 
      acetylcholine stimulation.
FAU - Koyama, H
AU  - Koyama H
AD  - Laboratory of Biomolecular Science, School of Pharmaceutical Sciences, Kitasato 
      University, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, Japan.
FAU - Adachi, M
AU  - Adachi M
FAU - Sekine, M
AU  - Sekine M
FAU - Katane, M
AU  - Katane M
FAU - Furuchi, T
AU  - Furuchi T
FAU - Homma, H
AU  - Homma H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060104
PL  - United States
TA  - Arch Biochem Biophys
JT  - Archives of biochemistry and biophysics
JID - 0372430
RN  - 0 (Calcium Channel Blockers)
RN  - 0 (Calcium Channels)
RN  - 0 (SNARE Proteins)
RN  - 4SR0Q8YD1X (D-Aspartic Acid)
RN  - I9ZF7L6G2L (Nifedipine)
RN  - N9YNS0M02X (Acetylcholine)
RN  - VTD58H1Z2X (Dopamine)
SB  - IM
MH  - Acetylcholine/pharmacology
MH  - Animals
MH  - Biological Transport, Active
MH  - Calcium Channel Blockers/pharmacology
MH  - Calcium Channels/physiology
MH  - Cytoplasmic Vesicles/metabolism
MH  - Cytosol/*metabolism
MH  - D-Aspartic Acid/*metabolism
MH  - Dopamine/metabolism
MH  - Exocytosis
MH  - Ion Channel Gating
MH  - Nifedipine/pharmacology
MH  - Osmolar Concentration
MH  - PC12 Cells
MH  - Rats
MH  - SNARE Proteins/physiology
EDAT- 2006/01/24 09:00
MHDA- 2006/04/15 09:00
CRDT- 2006/01/24 09:00
PHST- 2005/11/01 00:00 [received]
PHST- 2005/12/09 00:00 [revised]
PHST- 2005/12/15 00:00 [accepted]
PHST- 2006/01/24 09:00 [pubmed]
PHST- 2006/04/15 09:00 [medline]
PHST- 2006/01/24 09:00 [entrez]
AID - S0003-9861(05)00513-8 [pii]
AID - 10.1016/j.abb.2005.12.008 [doi]
PST - ppublish
SO  - Arch Biochem Biophys. 2006 Feb 15;446(2):131-9. doi: 10.1016/j.abb.2005.12.008. 
      Epub 2006 Jan 4.