PMID- 16427805
OWN - NLM
STAT- MEDLINE
DCOM- 20060522
LR  - 20191210
IS  - 1434-4610 (Print)
IS  - 1434-4610 (Linking)
VI  - 157
IP  - 1
DP  - 2006 Feb
TI  - Fluorescence in situ hybridization with specific oligonucleotide rRNA probes 
      distinguishes the sibling species Stylonychia lemnae and Stylonychia mytilus 
      (Ciliophora, Spirotrichea).
PG  - 21-30
AB  - Based on morphological and morphogenetic characters alone, the sibling species 
      Stylonychia lemnae and Stylonychia mytilus, members of the Stylonychia mytilus 
      complex, can hardly be distinguished. However, biochemical investigations of the 
      isoenzyme pattern of different enzymes showed a distinct differentiation between 
      these two species. In the last few years, fluorescence in situ hybridization 
      (FISH) techniques have become a suitable and reliable tool for identification and 
      differentiation of closely related species of protozoa, such as ciliates. To 
      distinguish the sibling species, a set of specific oligonucleotide probes were 
      developed. In the present study, the SSU rDNA of 7 clones of Stylonychia lemnae 
      and 13 clones of Stylonychia mytilus, isolated from different geographic regions, 
      were sequenced. Comparing all SSU rDNA sequences of both species, only one single 
      difference within the whole gene was detected. Based on this difference, a set of 
      two oligonucleotide probes, targeting the SSU rRNA of each species (Stylonychia 
      mytilus and Stylonychia lemnae) was designed. These probes were successfully 
      tested by applying the FISH techniques on preserved cells of different clones of 
      both species.
FAU - Schmidt, Stephanie L
AU  - Schmidt SL
AD  - Institute of Biology II, Department of Molecular Evolution and Animal 
      Systematics, University of Leipzig, Talstrasse 33, 04103 Leipzig, Germany. 
      sschmidt@rz.uni-leipzig.de
FAU - Bernhard, Detlef
AU  - Bernhard D
FAU - Schlegel, Martin
AU  - Schlegel M
FAU - Fried, Johannes
AU  - Fried J
LA  - eng
SI  - GENBANK/AM086648
SI  - GENBANK/AM086649
SI  - GENBANK/AM086650
SI  - GENBANK/AM086651
SI  - GENBANK/AM086652
SI  - GENBANK/AM086653
SI  - GENBANK/AM086654
SI  - GENBANK/AM086655
SI  - GENBANK/AM086656
SI  - GENBANK/AM086657
SI  - GENBANK/AM086658
SI  - GENBANK/AM086659
SI  - GENBANK/AM086660
SI  - GENBANK/AM086661
SI  - GENBANK/AM086662
SI  - GENBANK/AM086663
SI  - GENBANK/AM086664
SI  - GENBANK/AM086665
SI  - GENBANK/AM086666
SI  - GENBANK/AM086667
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060120
PL  - Germany
TA  - Protist
JT  - Protist
JID - 9806488
RN  - 0 (DNA, Protozoan)
RN  - 0 (Oligonucleotide Probes)
RN  - 0 (RNA, Ribosomal)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Ciliophora/*classification/genetics
MH  - DNA, Protozoan/analysis
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Molecular Sequence Data
MH  - *Oligonucleotide Probes
MH  - RNA, Ribosomal/*genetics
MH  - Sequence Analysis, DNA
MH  - Species Specificity
EDAT- 2006/01/24 09:00
MHDA- 2006/05/23 09:00
CRDT- 2006/01/24 09:00
PHST- 2005/06/20 00:00 [received]
PHST- 2005/09/27 00:00 [accepted]
PHST- 2006/01/24 09:00 [pubmed]
PHST- 2006/05/23 09:00 [medline]
PHST- 2006/01/24 09:00 [entrez]
AID - S1434-4610(05)00102-1 [pii]
AID - 10.1016/j.protis.2005.09.004 [doi]
PST - ppublish
SO  - Protist. 2006 Feb;157(1):21-30. doi: 10.1016/j.protis.2005.09.004. Epub 2006 Jan 
      20.