PMID- 16428107
OWN - NLM
STAT- MEDLINE
DCOM- 20060524
LR  - 20131121
IS  - 0007-1668 (Print)
IS  - 0007-1668 (Linking)
VI  - 46
IP  - 6
DP  - 2005 Dec
TI  - Quantitative analysis of embryonic kidney impairment by confocal microscopy and 
      stereology: effect of 1,2-dibromoethane in the chick mesonephros.
PG  - 661-7
AB  - 1. Chick embryos in ovo were treated with a teratogenic dose of 1,2-dibromoethane 
      (DBE) on embryonic day (ED) 3. On ED 6 and 10, histological sections of whole 
      embryos were prepared for confocal microscopy. In parallel, mesonephroi of 
      10-d-old embryos were dissected for in situ staining with acridine orange (AO), a 
      fluorescence probe for lysosomes. 2. DBE impaired differentiation of renal 
      vessels which manifested as a delay in rearrangement of primitive renal vascular 
      architecture on ED 6 and a significant reduction of the mesonephric 
      vascularisation on ED 10. This was accompanied by delayed functional maturation 
      of embryonic kidney, as suggested by staining with AO. 3. Renal vessels appeared 
      to be more susceptible to DBE than tubules. Unequal growth of these renal 
      components might be a cause of DBE-induced spatial disorganisation of tubular 
      apparatus. 4. Nephrotoxic effects of DBE during the embryonic period are 
      associated primarily with damage to the renal blood supply. 5. Confocal 
      microscopy, stereological methods and three-dimensional reconstruction of 
      developing tissues are useful tools to investigate pathogenic processes during 
      embryonic development.
FAU - Naprstkova, I
AU  - Naprstkova I
AD  - Institute of Physiology, Academy of Sciences of the Czech Republic.
FAU - Radochova, B
AU  - Radochova B
FAU - Novotna, B
AU  - Novotna B
FAU - Jirkovska, M
AU  - Jirkovska M
FAU - Janacek, J
AU  - Janacek J
FAU - Kubinova, L
AU  - Kubinova L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Br Poult Sci
JT  - British poultry science
JID - 15740290R
RN  - 1N41638RNO (Ethylene Dibromide)
SB  - IM
MH  - Animals
MH  - Chick Embryo
MH  - Ethylene Dibromide/*pharmacology
MH  - Kidney/*drug effects/*embryology/pathology
MH  - Mesonephros/*drug effects/growth & development/pathology
MH  - Microscopy, Confocal
EDAT- 2006/01/24 09:00
MHDA- 2006/05/25 09:00
CRDT- 2006/01/24 09:00
PHST- 2006/01/24 09:00 [pubmed]
PHST- 2006/05/25 09:00 [medline]
PHST- 2006/01/24 09:00 [entrez]
AID - T52585251X266617 [pii]
AID - 10.1080/00071660500394064 [doi]
PST - ppublish
SO  - Br Poult Sci. 2005 Dec;46(6):661-7. doi: 10.1080/00071660500394064.