PMID- 16428241
OWN - NLM
STAT- MEDLINE
DCOM- 20060621
LR  - 20210217
IS  - 1535-9476 (Print)
IS  - 1535-9476 (Linking)
VI  - 5
IP  - 4
DP  - 2006 Apr
TI  - High expression of antioxidant proteins in dendritic cells: possible implications 
      in atherosclerosis.
PG  - 726-36
AB  - Dendritic cells (DCs) display the unique ability to activate naive T cells and to 
      initiate primary T cell responses revealed in DC-T cell alloreactions. DCs 
      frequently operate under stress conditions. Oxidative stress enhances the 
      production of inflammatory cytokines by DCs. We performed a proteomic analysis to 
      see which major changes occur, at the protein expression level, during DC 
      differentiation and maturation. Comparative two-dimensional gel analysis of the 
      monocyte, immature DC, and mature DC stages was performed. Manganese superoxide 
      dismutase (Mn-SOD) reached 0.7% of the gel-displayed proteins at the mature DC 
      stage. This important amount of Mn-SOD is a primary antioxidant defense system 
      against superoxide radicals, but its product, H(2)O(2), is also deleterious for 
      cells. Peroxiredoxin (Prx) enzymes play an important role in eliminating such 
      peroxide. Prx1 expression level continuously increased during DC differentiation 
      and maturation, whereas Prx6 continuously decreased, and Prx2 peaked at the 
      immature DC stage. As a consequence, DCs were more resistant than monocytes to 
      apoptosis induced by high amounts of oxidized low density lipoproteins containing 
      toxic organic peroxides and hydrogen peroxide. Furthermore DC-stimulated T cells 
      produced high levels of receptor activator of nuclear factor kappaB ligand, a 
      chemotactic and survival factor for monocytes and DCs. This study provides 
      insights into the original ability of DCs to express very high levels of 
      antioxidant enzymes such as Mn-SOD and Prx1, to detoxify oxidized low density 
      lipoproteins, and to induce high levels of receptor activator of nuclear factor 
      kappaB ligand by the T cells they activate and further emphasizes the role that 
      DCs might play in atherosclerosis, a pathology recognized as a chronic 
      inflammatory disorder.
FAU - Rivollier, Aymeric
AU  - Rivollier A
AD  - INSERM U503, Universite Lyon 1, IFR128-Biosciences Gerland, 21 avenue Tony 
      Garnier, 69 007 Lyon, France.
FAU - Perrin-Cocon, Laure
AU  - Perrin-Cocon L
FAU - Luche, Sylvie
AU  - Luche S
FAU - Diemer, Helene
AU  - Diemer H
FAU - Strub, Jean-Marc
AU  - Strub JM
FAU - Hanau, Daniel
AU  - Hanau D
FAU - van Dorsselaer, Alain
AU  - van Dorsselaer A
FAU - Lotteau, Vincent
AU  - Lotteau V
FAU - Rabourdin-Combe, Chantal
AU  - Rabourdin-Combe C
FAU - Rabilloud, Thierry
AU  - Rabilloud T
FAU - Servet-Delprat, Christine
AU  - Servet-Delprat C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060119
PL  - United States
TA  - Mol Cell Proteomics
JT  - Molecular & cellular proteomics : MCP
JID - 101125647
RN  - 0 (Antioxidants)
RN  - 0 (Carrier Proteins)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (RANK Ligand)
RN  - 0 (Receptor Activator of Nuclear Factor-kappa B)
RN  - 0 (TNFRSF11A protein, human)
RN  - 0 (TNFSF11 protein, human)
RN  - EC 1.11.1.- (Peroxidases)
RN  - EC 1.11.1.15 (Peroxiredoxins)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
SB  - IM
MH  - Adult
MH  - Antioxidants/*metabolism
MH  - Atherosclerosis/*metabolism
MH  - Blotting, Western
MH  - Carrier Proteins/metabolism
MH  - Coculture Techniques
MH  - Dendritic Cells/enzymology/*metabolism
MH  - Electrophoresis, Gel, Two-Dimensional
MH  - Flow Cytometry
MH  - Fluorescent Antibody Technique
MH  - Humans
MH  - Membrane Glycoproteins/metabolism
MH  - Peroxidases/metabolism
MH  - Peroxiredoxins
MH  - RANK Ligand
MH  - Receptor Activator of Nuclear Factor-kappa B
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
MH  - Superoxide Dismutase/metabolism
EDAT- 2006/01/24 09:00
MHDA- 2006/06/22 09:00
CRDT- 2006/01/24 09:00
PHST- 2006/01/24 09:00 [pubmed]
PHST- 2006/06/22 09:00 [medline]
PHST- 2006/01/24 09:00 [entrez]
AID - S1535-9476(20)30356-X [pii]
AID - 10.1074/mcp.M500262-MCP200 [doi]
PST - ppublish
SO  - Mol Cell Proteomics. 2006 Apr;5(4):726-36. doi: 10.1074/mcp.M500262-MCP200. Epub 
      2006 Jan 19.