PMID- 16429448
OWN - NLM
STAT- MEDLINE
DCOM- 20060517
LR  - 20220410
IS  - 0360-4012 (Print)
IS  - 0360-4012 (Linking)
VI  - 83
IP  - 4
DP  - 2006 Mar
TI  - Proinflammatory cytokines expression in noise-induced damaged cochlea.
PG  - 575-83
AB  - Recent studies have showed that inflammatory responses occur in inner ear under 
      various damaging conditions including noise-overstimulation. We evaluated the 
      time-dependent expression of proinflammatory cytokines in noise-exposed rat 
      cochlea. Among several detected cytokines, real-time RT-PCR showed that 
      interleukin-1beta (IL-1beta) and interleukin-6 (IL-6) were significantly induced 
      3 hr after noise exposure, and quickly downregulated to the basal level. Tumor 
      necrosis factor-alpha (TNF-alpha) was also slightly upregulated immediately after 
      noise exposure. Immunohistochemical analysis showed that IL-6 expression was 
      distinctively induced within the lateral side of the spiral ligament. Sequential 
      expression analysis showed that IL-6 immunoreactivity was initially found in the 
      cytoplasm of lateral wall cells, including Type IV and III fibrocytes, and 
      expanded broader throughout the lateral wall, finally to the stria vascularis. 
      Because of the negative Iba-1 staining, IL-6 expression in the early-phase was 
      not due to macrophage or microglia activation. IL-6 was also detected in spiral 
      ganglion neurons at 12 and 24 hr after noise exposure. Our data demonstrates the 
      production of proinflammatory cytokines, including TNF-alpha, IL-1beta, and IL-6, 
      in early phase of noise overstimulated cochlea. IL-6 expression was observed in 
      the spiral ligament, stria vascularis, and spiral ganglion neurons. These 
      cytokines, produced by the cochlear structure itself in response to noise 
      exposure, may initiate an inflammatory response and have some role in the 
      mechanism of noise-induced cochlear damage.
CI  - Copyright 2006 Wiley-Liss, Inc.
FAU - Fujioka, Masato
AU  - Fujioka M
AD  - Department of Otolaryngology, Head and Neck Surgery, Keio University School of 
      Medicine, Tokyo, Japan.
FAU - Kanzaki, Sho
AU  - Kanzaki S
FAU - Okano, Hirotaka James
AU  - Okano HJ
FAU - Masuda, Masatsugu
AU  - Masuda M
FAU - Ogawa, Kaoru
AU  - Ogawa K
FAU - Okano, Hideyuki
AU  - Okano H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Neurosci Res
JT  - Journal of neuroscience research
JID - 7600111
RN  - 0 (Cytokines)
RN  - 0 (Interleukin-1)
RN  - 0 (Interleukin-6)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Cochlea/*injuries/*metabolism/pathology
MH  - Cytokines/*biosynthesis
MH  - Evoked Potentials, Auditory, Brain Stem/physiology
MH  - Hearing Loss, Noise-Induced/*metabolism/pathology
MH  - Immunohistochemistry
MH  - Interleukin-1/biosynthesis
MH  - Interleukin-6/biosynthesis
MH  - Male
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Tumor Necrosis Factor-alpha/biosynthesis
EDAT- 2006/01/24 09:00
MHDA- 2006/05/18 09:00
CRDT- 2006/01/24 09:00
PHST- 2006/01/24 09:00 [pubmed]
PHST- 2006/05/18 09:00 [medline]
PHST- 2006/01/24 09:00 [entrez]
AID - 10.1002/jnr.20764 [doi]
PST - ppublish
SO  - J Neurosci Res. 2006 Mar;83(4):575-83. doi: 10.1002/jnr.20764.