PMID- 16430499
OWN - NLM
STAT- MEDLINE
DCOM- 20060918
LR  - 20060124
IS  - 1364-5072 (Print)
IS  - 1364-5072 (Linking)
VI  - 100
IP  - 2
DP  - 2006 Feb
TI  - Which are the polyphosphate accumulating organisms in full-scale activated sludge 
      enhanced biological phosphate removal systems in Australia?
PG  - 233-43
AB  - AIMS: To see if the compositions of the microbial communities in full scale 
      enhanced biological phosphorus removal activated sludge systems were the same as 
      those from laboratory scale sequencing batch reactors fed a synthetic sewage. 
      METHODS: Biomass samples taken from nine full scale enhanced biological phosphate 
      removal (EBPR) activated sludge plants in the eastern states of Australia were 
      analysed for their populations of polyphosphate (polyP)-accumulating organisms 
      (PAO) using semi-quantitative fluorescence in situ hybridization (FISH) in 
      combination with DAPI (4'-6-diamidino-2-phenylindole) staining for polyP. 
      RESULTS: Very few betaproteobacterial Rhodocyclus related organisms could be 
      detected by FISH in most of the plants examined, and even where present, not all 
      these cells even within a single cluster, stained positively for polyP with DAPI. 
      In some plants in samples from aerobic reactors the Actinobacteria dominated 
      populations containing polyP. CONCLUSIONS: The PAO populations in full-scale EBPR 
      systems often differ to those seen in laboratory scale reactors fed artificial 
      sewage, and Rhodocyclus related organisms, dominating these latter communities 
      may not be as important in full-scale systems. Instead Actinobacteria may be the 
      major PAO. SIGNIFICANCE AND IMPACT OF THE STUDY: These findings illustrate how 
      little is still known about the microbial ecology of EBPR processes and that more 
      emphasis should now be placed on analysis of full-scale plants if microbiological 
      methods are to be applied to monitoring their performances.
FAU - Beer, M
AU  - Beer M
AD  - Biotechnology Research Centre, La Trobe University, Bendigo, Vic., Australia.
FAU - Stratton, H M
AU  - Stratton HM
FAU - Griffiths, P C
AU  - Griffiths PC
FAU - Seviour, R J
AU  - Seviour RJ
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Appl Microbiol
JT  - Journal of applied microbiology
JID - 9706280
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Indoles)
RN  - 0 (Polyphosphates)
RN  - 0 (Sewage)
RN  - 47165-04-8 (DAPI)
SB  - IM
MH  - Actinobacteria/isolation & purification
MH  - Bacteria/*isolation & purification
MH  - Biomass
MH  - Fluorescent Dyes/analysis
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Indoles/analysis
MH  - Polyphosphates/*metabolism
MH  - Proteobacteria/isolation & purification
MH  - Refuse Disposal/*methods
MH  - Rhodocyclaceae/isolation & purification
MH  - Sewage/*microbiology
EDAT- 2006/01/25 09:00
MHDA- 2006/09/19 09:00
CRDT- 2006/01/25 09:00
PHST- 2006/01/25 09:00 [pubmed]
PHST- 2006/09/19 09:00 [medline]
PHST- 2006/01/25 09:00 [entrez]
AID - JAM2784 [pii]
AID - 10.1111/j.1365-2672.2005.02784.x [doi]
PST - ppublish
SO  - J Appl Microbiol. 2006 Feb;100(2):233-43. doi: 10.1111/j.1365-2672.2005.02784.x.