PMID- 16432012
OWN - NLM
STAT- MEDLINE
DCOM- 20060321
LR  - 20201006
IS  - 0022-1317 (Print)
IS  - 0022-1317 (Linking)
VI  - 87
IP  - Pt 2
DP  - 2006 Feb
TI  - Human herpesvirus 6 envelope cholesterol is required for virus entry.
PG  - 277-285
LID - 10.1099/vir.0.81551-0 [doi]
AB  - In this study, the role of cholesterol in the envelope of human herpesvirus 6 
      (HHV-6) was examined by using methyl-beta-cyclodextrin (MbetaCD) depletion. When 
      cholesterol was removed from HHV-6 virions with MbetaCD, infectivity was 
      abolished, but it could be rescued by the addition of exogenous cholesterol. 
      HHV-6 binding was affected slightly by MbetaCD treatment. In contrast, envelope 
      cholesterol depletion markedly affected HHV-6 infectivity and HHV-6-induced cell 
      fusion. These results suggest that the cholesterol present in the HHV-6 envelope 
      plays a prominent role in the fusion process and is a key component in viral 
      entry.
FAU - Huang, Honglan
AU  - Huang H
AD  - Department of Microbiology, Osaka University, Graduate School of Medicine, Suita, 
      Osaka 565-0871, Japan.
FAU - Li, Yongmei
AU  - Li Y
AD  - Department of Microbiology, Osaka University, Graduate School of Medicine, Suita, 
      Osaka 565-0871, Japan.
FAU - Sadaoka, Tomohiko
AU  - Sadaoka T
AD  - Laboratory of Virology and Vaccinology, Division of Biomedical Research, National 
      Institute of Biomedical Innovation, 7-6-8 Saito-Asagi, Ibaraki, Osaka 567-0085, 
      Japan.
FAU - Tang, Huanmin
AU  - Tang H
AD  - Laboratory of Virology and Vaccinology, Division of Biomedical Research, National 
      Institute of Biomedical Innovation, 7-6-8 Saito-Asagi, Ibaraki, Osaka 567-0085, 
      Japan.
FAU - Yamamoto, Takahito
AU  - Yamamoto T
AD  - The Research Foundation for Microbial Diseases of Osaka University, 2-9-41 
      Yahata-Cho, Kanonji, Kagawa 768-0061, Japan.
FAU - Yamanishi, Koichi
AU  - Yamanishi K
AD  - Laboratory of Virology and Vaccinology, Division of Biomedical Research, National 
      Institute of Biomedical Innovation, 7-6-8 Saito-Asagi, Ibaraki, Osaka 567-0085, 
      Japan.
AD  - Department of Microbiology, Osaka University, Graduate School of Medicine, Suita, 
      Osaka 565-0871, Japan.
FAU - Mori, Yasuko
AU  - Mori Y
AD  - Laboratory of Virology and Vaccinology, Division of Biomedical Research, National 
      Institute of Biomedical Innovation, 7-6-8 Saito-Asagi, Ibaraki, Osaka 567-0085, 
      Japan.
AD  - Department of Microbiology, Osaka University, Graduate School of Medicine, Suita, 
      Osaka 565-0871, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Gen Virol
JT  - The Journal of general virology
JID - 0077340
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (beta-Cyclodextrins)
RN  - 0 (methyl-beta-cyclodextrin)
RN  - 97C5T2UQ7J (Cholesterol)
SB  - IM
MH  - Cholesterol/*physiology
MH  - Herpesvirus 6, Human/chemistry/drug effects/*physiology
MH  - Humans
MH  - Membrane Glycoproteins/chemistry/genetics/*metabolism
MH  - Viral Envelope Proteins/metabolism
MH  - Virion/metabolism
MH  - beta-Cyclodextrins/pharmacology
EDAT- 2006/01/25 09:00
MHDA- 2006/03/22 09:00
CRDT- 2006/01/25 09:00
PHST- 2006/01/25 09:00 [pubmed]
PHST- 2006/03/22 09:00 [medline]
PHST- 2006/01/25 09:00 [entrez]
AID - 10.1099/vir.0.81551-0 [doi]
PST - ppublish
SO  - J Gen Virol. 2006 Feb;87(Pt 2):277-285. doi: 10.1099/vir.0.81551-0.