PMID- 16433684
OWN - NLM
STAT- MEDLINE
DCOM- 20060327
LR  - 20060125
IS  - 0906-6705 (Print)
IS  - 0906-6705 (Linking)
VI  - 15
IP  - 2
DP  - 2006 Feb
TI  - Role of microchimerism in the pathogenesis of oral lichen planus.
PG  - 125-9
AB  - OBJECTIVE: Microchimerism of persistent fetal cells has been implicated in some 
      cell-mediated autoimmune diseases. This study examines the hypothesis that fetal 
      microchimerism plays a role in the pathogenesis of lichen planus (LP) affecting 
      the oral cavity. STUDY DESIGN: Mucosal biopsies of 12 women with oral LP (OLP) 
      were tested for the presence of both male cells and male DNA originating from 
      prior pregnancies or prior blood transfusions. Six male patients with OLP served 
      as a control group. Biopsies were analyzed for the presence of 
      Y-chromosome-positive cells by fluorescence in situ hybridization (FISH) with X- 
      and Y-specific DNA probes. To confirm the FISH findings, we used fluorescent 
      polymerase chain reaction (PCR) to identify Y-chromosome sequences in DNA 
      extracted from mucosal lesions. RESULTS: Using FISH technology, all the 18 biopsy 
      samples showed good hybridization results. In females, Y-chromosome-specific 
      signals were not detected by FISH at any site of the lesions. PCR amplification 
      demonstrated a single peak at the locus specific for the X-chromosome. 
      CONCLUSION: Male DNA microchimerism was not present in any of the investigated 
      lesions, suggesting that microchimerism because of persisting male fetal cells is 
      unlikely to play a major role in the pathogenesis of OLP.
FAU - Weger, W
AU  - Weger W
AD  - Department of Dermatology, Medical University of Graz, Austria.
FAU - Bauer, M
AU  - Bauer M
FAU - Odell, E
AU  - Odell E
FAU - Pertl, B
AU  - Pertl B
FAU - Cerroni, L
AU  - Cerroni L
FAU - Kerl, H
AU  - Kerl H
FAU - Jakse, N
AU  - Jakse N
FAU - Pertl, C
AU  - Pertl C
LA  - eng
PT  - Journal Article
PL  - Denmark
TA  - Exp Dermatol
JT  - Experimental dermatology
JID - 9301549
RN  - 0 (DNA Probes)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Base Sequence
MH  - Case-Control Studies
MH  - *Chimerism
MH  - Chromosomes, Human, X/genetics
MH  - Chromosomes, Human, Y/genetics
MH  - DNA Probes/genetics
MH  - Female
MH  - Fetomaternal Transfusion
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Lichen Planus, Oral/*etiology/genetics/immunology
MH  - Male
MH  - Middle Aged
MH  - Pregnancy
EDAT- 2006/01/26 09:00
MHDA- 2006/03/28 09:00
CRDT- 2006/01/26 09:00
PHST- 2006/01/26 09:00 [pubmed]
PHST- 2006/03/28 09:00 [medline]
PHST- 2006/01/26 09:00 [entrez]
AID - EXD393 [pii]
AID - 10.1111/j.1600-0625.2006.00393.x [doi]
PST - ppublish
SO  - Exp Dermatol. 2006 Feb;15(2):125-9. doi: 10.1111/j.1600-0625.2006.00393.x.