PMID- 16434311
OWN - NLM
STAT- MEDLINE
DCOM- 20060310
LR  - 20061115
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 164
IP  - 2
DP  - 2006 Jan 15
TI  - Distribution of breakpoints on chromosome 18 in breast, colorectal, and 
      pancreatic carcinoma cell lines.
PG  - 97-109
AB  - Chromosome 18 is frequently rearranged in carcinomas. We explored the 
      distribution of breakpoints affecting chromosome 18 by mapping 56 breakpoints in 
      26 carcinoma cell lines by fluorescence in situ hybridization (FISH) using 
      bacterial artificial chromosomes (BACs) and band paints. The distribution of 
      breaks among 18 intervals of chromosome 18 was significantly nonrandom. The 
      interval spanning the centromere contained the greatest number of breaks and had 
      the highest average copy number of any interval. There was a high density of 
      breaks close to the centromere as well as actually within the centromere. A 
      cluster of breaks encompassing SMAD4 was associated with the minimum average copy 
      number, consistent with SMAD4 being a tumor suppressor gene. There may be another 
      cluster of breaks around 18q12. We offer two interpretations of the concentration 
      of breaks near the centromere. It may reflect selection for an oncogene near the 
      centromere, or there may be an underlying bias of breakage toward the centromere. 
      We show that the latter is predicted by a simple model that invokes random 
      breakage following anchorage of some random point on the chromosome, or selection 
      of breaks proximal to one of several tumor suppressor genes.
FAU - Alsop, Amber E
AU  - Alsop AE
AD  - Cancer Genomics Program, Hutchison-MRC Research Centre, Department of Pathology 
      and Oncology, University of Cambridge, Hills Road, Cambridge CB2 2XZ, United 
      Kingdom.
FAU - Teschendorff, Andrew E
AU  - Teschendorff AE
FAU - Edwards, Paul A W
AU  - Edwards PA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - Breast Neoplasms/*genetics
MH  - Cell Line, Tumor
MH  - *Chromosome Breakage
MH  - Chromosomes, Artificial, Bacterial
MH  - *Chromosomes, Human, Pair 18
MH  - Colorectal Neoplasms/*genetics
MH  - Female
MH  - Gene Dosage
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Pancreatic Neoplasms/*genetics
EDAT- 2006/01/26 09:00
MHDA- 2006/03/11 09:00
CRDT- 2006/01/26 09:00
PHST- 2005/08/08 00:00 [received]
PHST- 2005/09/20 00:00 [revised]
PHST- 2005/09/22 00:00 [accepted]
PHST- 2006/01/26 09:00 [pubmed]
PHST- 2006/03/11 09:00 [medline]
PHST- 2006/01/26 09:00 [entrez]
AID - S0165-4608(05)00557-1 [pii]
AID - 10.1016/j.cancergencyto.2005.09.011 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2006 Jan 15;164(2):97-109. doi: 
      10.1016/j.cancergencyto.2005.09.011.