PMID- 16434960
OWN - NLM
STAT- MEDLINE
DCOM- 20060501
LR  - 20131121
IS  - 1465-7392 (Print)
IS  - 1465-7392 (Linking)
VI  - 8
IP  - 3
DP  - 2006 Mar
TI  - Transient colocalization of X-inactivation centres accompanies the initiation of 
      X inactivation.
PG  - 293-9
AB  - The initial differential treatment of the two X chromosomes during X-chromosome 
      inactivation is controlled by the X-inactivation centre (Xic). This locus 
      determines how many X chromosomes are present in a cell ('counting') and which X 
      chromosome will be inactivated in female cells ('choice'). Critical control 
      sequences in the Xic include the non-coding RNAs Xist and Tsix, and long-range 
      chromatin elements. However, little is known about the process that ensures that 
      X inactivation is triggered appropriately when more than one Xic is present in a 
      cell. Using three-dimensional fluorescence in situ hybridization (FISH) analysis, 
      we showed that the two Xics transiently colocalize, just before X inactivation, 
      in differentiating female embryonic stem cells. Using Xic transgenes capable of 
      imprinted but not random X inactivation, and Xic deletions that disrupt random X 
      inactivation, we demonstrated that Xic colocalization is linked to Xic function 
      in random X inactivation. Both long-range sequences and the Tsix element, which 
      generates the antisense transcript to Xist, are required for the transient 
      interaction of Xics. We propose that transient colocalization of Xics may be 
      necessary for a cell to determine Xic number and to ensure the correct initiation 
      of X inactivation.
FAU - Bacher, Christian P
AU  - Bacher CP
AD  - German Cancer Research Center (DKFZ), Im Neuenheimer Feld 280, D-69120 
      Heidelberg, German.
FAU - Guggiari, Michele
AU  - Guggiari M
FAU - Brors, Benedikt
AU  - Brors B
FAU - Augui, Sandrine
AU  - Augui S
FAU - Clerc, Philippe
AU  - Clerc P
FAU - Avner, Philip
AU  - Avner P
FAU - Eils, Roland
AU  - Eils R
FAU - Heard, Edith
AU  - Heard E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060124
PL  - England
TA  - Nat Cell Biol
JT  - Nature cell biology
JID - 100890575
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (RNA, Untranslated)
RN  - 0 (XIST non-coding RNA)
SB  - IM
CIN - Nat Cell Biol. 2006 Mar;8(3):207-9. PMID: 16508671
MH  - Animals
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - Embryo, Mammalian/cytology
MH  - Female
MH  - *Genomic Imprinting
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Mice
MH  - RNA, Long Noncoding
MH  - RNA, Untranslated/genetics/*physiology
MH  - Stem Cells/*physiology
MH  - X Chromosome/*physiology
MH  - *X Chromosome Inactivation
EDAT- 2006/01/26 09:00
MHDA- 2006/05/02 09:00
CRDT- 2006/01/26 09:00
PHST- 2005/10/31 00:00 [received]
PHST- 2006/01/11 00:00 [accepted]
PHST- 2006/01/26 09:00 [pubmed]
PHST- 2006/05/02 09:00 [medline]
PHST- 2006/01/26 09:00 [entrez]
AID - ncb1365 [pii]
AID - 10.1038/ncb1365 [doi]
PST - ppublish
SO  - Nat Cell Biol. 2006 Mar;8(3):293-9. doi: 10.1038/ncb1365. Epub 2006 Jan 24.