PMID- 16436430
OWN - NLM
STAT- MEDLINE
DCOM- 20060609
LR  - 20200826
IS  - 1350-0872 (Print)
IS  - 1350-0872 (Linking)
VI  - 152
IP  - Pt 2
DP  - 2006 Feb
TI  - Putative glycogen-accumulating organisms belonging to the Alphaproteobacteria 
      identified through rRNA-based stable isotope probing.
PG  - 419-429
LID - 10.1099/mic.0.28445-0 [doi]
AB  - Deterioration of enhanced biological phosphorus removal (EBPR) has been linked to 
      the proliferation of glycogen-accumulating organisms (GAOs), but few organisms 
      possessing the GAO metabolic phenotype have been identified. An unidentified GAO 
      was highly enriched in a laboratory-scale bioreactor and attempts to identify 
      this organism using conventional 16S rRNA gene cloning had failed. Therefore, 
      rRNA-based stable isotope probing followed by full-cycle rRNA analysis was used 
      to specifically identify the putative GAOs based on their characteristic 
      metabolic phenotype. The study obtained sequences from a group of 
      Alphaproteobacteria not previously shown to possess the GAO phenotype, but 90 % 
      identical by 16S rRNA gene analysis to a phylogenetic clade containing cloned 
      sequences from putative GAOs and the isolate Defluvicoccus vanus. Fluorescence in 
      situ hybridization (FISH) probes (DF988 and DF1020) were designed to target the 
      new group and post-FISH chemical staining demonstrated anaerobic-aerobic cycling 
      of polyhydroxyalkanoates, as per the GAO phenotype. The successful use of probes 
      DF988 and DF1020 required the use of unlabelled helper probes which increased 
      probe signal intensity up to 6.6-fold, thus highlighting the utility of helper 
      probes in FISH. The new group constituted 33 % of all Bacteria in the lab-scale 
      bioreactor from which they were identified and were also abundant (51 and 55 % of 
      Bacteria) in two other similar bioreactors in which phosphorus removal had 
      deteriorated. Unlike the previously identified Defluvicoccus-related organisms, 
      the group identified in this study were also found in two full-scale treatment 
      plants performing EBPR, suggesting that this group may be industrially relevant.
FAU - Meyer, Rikke Louise
AU  - Meyer RL
AD  - Advanced Wastewater Management Centre, The University of Queensland, St Lucia, 
      QLD 4072, Australia.
FAU - Saunders, Aaron Marc
AU  - Saunders AM
AD  - Advanced Wastewater Management Centre, The University of Queensland, St Lucia, 
      QLD 4072, Australia.
FAU - Blackall, Linda Louise
AU  - Blackall LL
AD  - Advanced Wastewater Management Centre, The University of Queensland, St Lucia, 
      QLD 4072, Australia.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Microbiology (Reading)
JT  - Microbiology (Reading, England)
JID - 9430468
RN  - 0 (DNA, Bacterial)
RN  - 0 (Isotopes)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Sewage)
RN  - 9005-79-2 (Glycogen)
SB  - IM
MH  - Alphaproteobacteria/*classification/genetics/metabolism
MH  - Bioreactors
MH  - DNA, Bacterial/chemistry/isolation & purification
MH  - Glycogen/*metabolism
MH  - In Situ Hybridization, Fluorescence
MH  - Isotopes
MH  - RNA, Ribosomal, 16S/analysis/genetics
MH  - Sewage/*microbiology
MH  - Waste Disposal, Fluid
MH  - Water Microbiology
EDAT- 2006/01/27 09:00
MHDA- 2006/06/10 09:00
CRDT- 2006/01/27 09:00
PHST- 2006/01/27 09:00 [pubmed]
PHST- 2006/06/10 09:00 [medline]
PHST- 2006/01/27 09:00 [entrez]
AID - 10.1099/mic.0.28445-0 [doi]
PST - ppublish
SO  - Microbiology (Reading). 2006 Feb;152(Pt 2):419-429. doi: 10.1099/mic.0.28445-0.