PMID- 16436659
OWN - NLM
STAT- MEDLINE
DCOM- 20060321
LR  - 20190608
IS  - 0002-9440 (Print)
IS  - 1525-2191 (Electronic)
IS  - 0002-9440 (Linking)
VI  - 168
IP  - 2
DP  - 2006 Feb
TI  - Regulation of monocyte chemoattractant protein-1 expression by tumor necrosis 
      factor-alpha and interleukin-1beta in first trimester human decidual cells: 
      implications for preeclampsia.
PG  - 445-52
AB  - The current study describes a statistically significant increase in macrophages 
      (CD68-positive cells) in the decidua of preeclamptic patients. To elucidate the 
      regulation of this monocyte infiltration, expression of monocyte chemoattractant 
      protein-1 (MCP-1) was assessed in leukocyte-free first trimester decidual cells. 
      Confluent decidual cells were primed for 7 days in either estradiol or estradiol 
      plus medroxyprogesterone acetate to mimic the decidualizing steroidal milieu of 
      the luteal phase and early pregnancy. The medium was exchanged for a serum-free 
      defined medium containing corresponding steroids +/- tumor necrosis factor 
      (TNF)-alpha or interleukin (IL)-1beta. After 24 hours, enzyme-linked 
      immunosorbent assay measurements indicated that the addition of 
      medroxyprogesterone acetate did not affect MCP-1 output, whereas 10 ng/ml of 
      TNF-alpha or IL-1beta increased output by 83.5-fold +/- 20.6 and 103.1-fold +/- 
      14.7, respectively (mean +/- SEM, n = 8, P < 0.05). Concentration-response 
      comparisons revealed that even 0.01 ng/ml of TNF-alpha or IL-1beta elevated MCP-1 
      output by more than 15-fold. Western blotting confirmed the enzyme-linked 
      immunosorbent assay results, and quantitative reverse transcriptase-polymerase 
      chain reaction confirmed corresponding effects on MCP-1 mRNA levels. The current 
      study demonstrates that TNF-alpha and IL-1beta enhance MCP-1 in first trimester 
      decidua. This finding suggests a mechanism by which recruitment of excess 
      macrophages to the decidua impairs endovascular trophoblast invasion, the primary 
      placental defect of preeclampsia.
FAU - Lockwood, Charles J
AU  - Lockwood CJ
AD  - Department of Obstetrics, Gynecology, and Reproductive Sciences, Yale University 
      School of Medicine, New Haven, CT 06520-8063, USA.
FAU - Matta, Paul
AU  - Matta P
FAU - Krikun, Graciela
AU  - Krikun G
FAU - Koopman, Louise A
AU  - Koopman LA
FAU - Masch, Rachel
AU  - Masch R
FAU - Toti, Paolo
AU  - Toti P
FAU - Arcuri, Felice
AU  - Arcuri F
FAU - Huang, Se-Te Joseph
AU  - Huang ST
FAU - Funai, Edmund F
AU  - Funai EF
FAU - Schatz, Frederick
AU  - Schatz F
LA  - eng
GR  - R01 HL070004/HL/NHLBI NIH HHS/United States
GR  - 1 R01 HL070004-02/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - Am J Pathol
JT  - The American journal of pathology
JID - 0370502
RN  - 0 (Antineoplastic Agents, Hormonal)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Culture Media, Serum-Free)
RN  - 0 (Drug Combinations)
RN  - 0 (Interleukin-1)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 4TI98Z838E (Estradiol)
RN  - C2QI4IOI2G (Medroxyprogesterone Acetate)
SB  - IM
MH  - Antineoplastic Agents, Hormonal/pharmacology
MH  - Blotting, Western
MH  - Cells, Cultured
MH  - Chemokine CCL2/*metabolism
MH  - Culture Media, Serum-Free
MH  - Decidua/cytology/drug effects/*metabolism
MH  - Drug Combinations
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Estradiol/pharmacology
MH  - Female
MH  - Humans
MH  - Interleukin-1/*pharmacology
MH  - Luteal Phase/metabolism
MH  - Macrophages/metabolism
MH  - Medroxyprogesterone Acetate/pharmacology
MH  - Pre-Eclampsia/*metabolism/pathology
MH  - Pregnancy
MH  - Pregnancy Trimester, First/*metabolism
MH  - RNA, Messenger/genetics/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Trophoblasts/cytology/metabolism
MH  - Tumor Necrosis Factor-alpha/*pharmacology
PMC - PMC1606506
EDAT- 2006/01/27 09:00
MHDA- 2006/03/22 09:00
PMCR- 2006/08/01
CRDT- 2006/01/27 09:00
PHST- 2006/01/27 09:00 [pubmed]
PHST- 2006/03/22 09:00 [medline]
PHST- 2006/01/27 09:00 [entrez]
PHST- 2006/08/01 00:00 [pmc-release]
AID - S0002-9440(10)62105-0 [pii]
AID - 10.2353/ajpath.2006.050082 [doi]
PST - ppublish
SO  - Am J Pathol. 2006 Feb;168(2):445-52. doi: 10.2353/ajpath.2006.050082.