PMID- 16439258
OWN - NLM
STAT- MEDLINE
DCOM- 20060328
LR  - 20161124
IS  - 0021-9673 (Print)
IS  - 0021-9673 (Linking)
VI  - 1105
IP  - 1-2
DP  - 2006 Feb 10
TI  - Determination of aristolochic acid in Chinese herbal medicine by capillary 
      electrophoresis with laser-induced fluorescence detection.
PG  - 127-34
AB  - We have demonstrated the analysis of aristolochic acids (AAs) that are naturally 
      occurring nephrotoxin and carcinogen by capillary electrophoresis in conjunction 
      with laser-induced fluorescence detection (CE-LIF). Owing to lack of intrinsic 
      fluorescence characteristics of oxidized AAs (OAAs), reduction of the analytes by 
      iron powder in 10.0 mM HCl is required prior to CE analysis. The reduced AAs 
      (RAAs) exhibit fluorescence at 477 nm when excited at 405 nm using a solid-state 
      blue laser. By using 50.0 mM sodium tetraborate (pH 9.0) containing 10.0 mM SDS, 
      the determination of AA-I and AA-II by CE-LIF has been achieved within 12 min. 
      The CE-LIF provides the LODs of 8.2 and 5.4 nM for AA-I and AA-II, respectively. 
      The simple CE-LIF method has been validated by the analysis of 61 Chinese herbal 
      samples. Prior to CE analysis, OAAs were extracted by using 5.0 mL MeOH, and then 
      the extracts were subjected to centrifugation at 3,000 rpm for 5 min. After 
      reduction, extraction, and centrifugation, the supernatants were collected and 
      subjected to CE analysis. Of the 61 samples, 14 samples contain AA-I and AA-II, 
      as well as 10 samples contain either AAI or AAII. The relative standard deviation 
      (RSD) values of the migration times for AA-I and AA-II are less than 2.5% and 
      2.1% for three consecutive measurements of each sample. The RSD values for the 
      peak heights corresponding to AA-I and AA-II in most samples are about 8.0% and 
      10.0%, respectively. The result shows that the present CE-LIF approach is 
      sensitive, simple, efficient, and accurate for the determination of AAs in real 
      samples.
FAU - Hsieh, Shih-Chung
AU  - Hsieh SC
AD  - Division of Nephrology, Shin Kong Wu Ho-Su Memorial Hospital, Taipei, Taiwan.
FAU - Huang, Ming-Feng
AU  - Huang MF
FAU - Lin, Bing-Shi
AU  - Lin BS
FAU - Chang, Huan-Tsung
AU  - Chang HT
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20050811
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 0 (Aristolochic Acids)
RN  - 0 (Drugs, Chinese Herbal)
RN  - 94218WFP5T (aristolochic acid I)
SB  - IM
MH  - Aristolochic Acids/*analysis/isolation & purification
MH  - Dietary Supplements/analysis
MH  - Drugs, Chinese Herbal/*chemistry
MH  - Electrophoresis, Capillary/*methods
MH  - Lasers
MH  - Oxidation-Reduction
MH  - Sensitivity and Specificity
MH  - Spectrometry, Fluorescence/methods
EDAT- 2006/01/28 09:00
MHDA- 2006/03/29 09:00
CRDT- 2006/01/28 09:00
PHST- 2005/05/17 00:00 [received]
PHST- 2005/07/13 00:00 [revised]
PHST- 2005/07/15 00:00 [accepted]
PHST- 2006/01/28 09:00 [pubmed]
PHST- 2006/03/29 09:00 [medline]
PHST- 2006/01/28 09:00 [entrez]
AID - S0021-9673(05)01558-X [pii]
AID - 10.1016/j.chroma.2005.07.056 [doi]
PST - ppublish
SO  - J Chromatogr A. 2006 Feb 10;1105(1-2):127-34. doi: 10.1016/j.chroma.2005.07.056. 
      Epub 2005 Aug 11.