PMID- 16439718 OWN - NLM STAT- MEDLINE DCOM- 20060622 LR - 20151119 IS - 1073-449X (Print) IS - 1073-449X (Linking) VI - 173 IP - 10 DP - 2006 May 15 TI - Protein profiles of bronchoalveolar lavage fluid from patients with pulmonary sarcoidosis. PG - 1145-54 AB - BACKGROUND: Pulmonary sarcoidosis is a multisystem granulomatous disease with various clinical phenotypes. So far, there has been little information on protein patterns (PPs) of bronchoalveolar lavage fluid (BALF) from patients with sarcoidosis and no data are available on PPs in clinical disease subtypes. OBJECTIVES: To investigate the PP of BALF from patients with pulmonary sarcoidosis, to evaluate whether PPs reflect disease course as assessed by chest X-ray (CXR), and to compare PPs between patients with/without Lofgren's syndrome. METHODS: Surface-enhanced laser desorption/ionization-time-of-flight mass spectroscopy was applied to investigate PPs in unconcentrated BALF from 65 patients (CXR stage I, n = 32; CXR stage II, n = 22, CXR stage III, n = 11) and 23 healthy control subjects. The Mann-Whitney U test was used to detect differentially expressed protein peaks. After reversed-phase fractionation, peptide fingerprint mapping and immunodepletion were used to identify deregulated (up-regulated or down-regulated) proteins. RESULTS: Forty differentially expressed protein entities (2.75-185.62 kD) were detected in patients with pulmonary sarcoidosis versus control subjects (p < 0.05). Whereas 13 peaks (33%) were present across all CXR stages, 27 (67%) were specific for particular CXR stages. Comparison of PPs between CXR stage I patients with or without Lofgren's syndrome revealed 25 differentially expressed peaks. The total number of deregulated peaks and also of those associated with sarcoidosis as a whole were markedly lower in patients with Lofgren's syndrome in comparison with other sarcoid phenotypes. Human serum albumin, alpha1-antitrypsin, and protocadherin-2 precursor were identified from sarcoidosis-associated PP. CONCLUSION: Surface-enhanced laser desorption/ionization-time-of-flight mass spectroscopy enables determination of protein patterns in sarcoid BALF and allows detection of protein patterns linked to a particular disease course. FAU - Kriegova, Eva AU - Kriegova E AD - Palacky University, Medical Faculty, I.P. Pavlova Str. 6, CZ-775 20 Olomouc, Czech Republic. FAU - Melle, Christian AU - Melle C FAU - Kolek, Vitezslav AU - Kolek V FAU - Hutyrova, Beata AU - Hutyrova B FAU - Mrazek, Frantisek AU - Mrazek F FAU - Bleul, Annett AU - Bleul A FAU - du Bois, Roland M AU - du Bois RM FAU - von Eggeling, Ferdinand AU - von Eggeling F FAU - Petrek, Martin AU - Petrek M LA - eng PT - Comparative Study PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20060126 PL - United States TA - Am J Respir Crit Care Med JT - American journal of respiratory and critical care medicine JID - 9421642 RN - 0 (Biomarkers) RN - 0 (Proteins) SB - IM CIN - Am J Respir Crit Care Med. 2006 May 15;173(10):1053-4. PMID: 16679442 MH - Adult MH - Aged MH - Biomarkers/analysis MH - Bronchoalveolar Lavage Fluid/*chemistry/cytology MH - Case-Control Studies MH - Disease Progression MH - Down-Regulation MH - Female MH - Humans MH - Macrophages, Alveolar/metabolism MH - Male MH - Middle Aged MH - Peptide Mapping MH - Probability MH - *Protein Array Analysis MH - Proteins/genetics/*metabolism MH - Reference Values MH - Respiratory Function Tests MH - Sarcoidosis, Pulmonary/*diagnosis/metabolism MH - Sensitivity and Specificity MH - Severity of Illness Index MH - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization MH - Statistics, Nonparametric MH - Up-Regulation EDAT- 2006/01/28 09:00 MHDA- 2006/06/23 09:00 CRDT- 2006/01/28 09:00 PHST- 2006/01/28 09:00 [pubmed] PHST- 2006/06/23 09:00 [medline] PHST- 2006/01/28 09:00 [entrez] AID - 200507-1126OC [pii] AID - 10.1164/rccm.200507-1126OC [doi] PST - ppublish SO - Am J Respir Crit Care Med. 2006 May 15;173(10):1145-54. doi: 10.1164/rccm.200507-1126OC. Epub 2006 Jan 26.