PMID- 16440596
OWN - NLM
STAT- MEDLINE
DCOM- 20060302
LR  - 20191109
IS  - 1138-7548 (Print)
IS  - 1138-7548 (Linking)
VI  - 61
IP  - 3
DP  - 2005 Sep
TI  - Influence of streptozotocin-induced diabetes on hexokinase activity of rat 
      salivary glands.
PG  - 421-7
AB  - The influence of diabetes on the enzyme hexokinase (HK) was examined in the 
      salivary glands of rats. Diabetes was induced by an intraperitoneal injection of 
      streptozotocin (60 mg/Kg body weight) in overnight fasted rats (180-200 g). The 
      animals were killed 48 hours and 30 days after the induction of diabetes and the 
      submandibular and parotid salivary glands extracted for use. Hyperglycemia was 
      evaluated by determining the blood sugar. The area occupied by each intralobular 
      component, acini, ducts, total parenchyma and stroma was measured, and no 
      differences were observed compared with control. In the soluble fraction of the 
      submandibular gland, no difference in the specific activity of HK was observed, 
      between the diabetic and control animals, however, the activity per gland and per 
      g of tissue showed lower values than control. The specific activity of the bound 
      form was reduced in the diabetic gland. The results obtained for the parotid 
      gland were different from the submandibular. The specific activity of both the 
      soluble and bound forms were increased in the diabetic animals. The 
      DEAE-cellulose column chromatography of the soluble and bound forms of the enzyme 
      from both glands showed a first peak appearing during the washing of the column 
      and two other peaks were eluted by the gradient. Thus, three isoenzymes in the 
      submandibular and parotid salivary glands for the control and diabetic rats have 
      been found.
FAU - Nogueira, F N
AU  - Nogueira FN
AD  - Oral Biology Research Center, Faculty of Dentistry, University of Sao Paulo, 
      Brazil.
FAU - Santos, M F dos
AU  - Santos MF
FAU - Nicolau, J
AU  - Nicolau J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Spain
TA  - J Physiol Biochem
JT  - Journal of physiology and biochemistry
JID - 9812509
RN  - 5W494URQ81 (Streptozocin)
RN  - EC 2.7.1.1 (Hexokinase)
SB  - IM
MH  - Animals
MH  - Chromatography, DEAE-Cellulose
MH  - Diabetes Mellitus, Experimental/*enzymology
MH  - Hexokinase/*metabolism
MH  - Male
MH  - Rats
MH  - Rats, Wistar
MH  - Salivary Glands/*enzymology
MH  - Streptozocin
EDAT- 2006/01/31 09:00
MHDA- 2006/03/03 09:00
CRDT- 2006/01/31 09:00
PHST- 2006/01/31 09:00 [pubmed]
PHST- 2006/03/03 09:00 [medline]
PHST- 2006/01/31 09:00 [entrez]
AID - 10.1007/BF03168448 [doi]
PST - ppublish
SO  - J Physiol Biochem. 2005 Sep;61(3):421-7. doi: 10.1007/BF03168448.