PMID- 16441371
OWN - NLM
STAT- MEDLINE
DCOM- 20060525
LR  - 20131121
IS  - 0266-8254 (Print)
IS  - 0266-8254 (Linking)
VI  - 42
IP  - 2
DP  - 2006 Feb
TI  - Mutagenic effect of acridine orange on the expression of penicillin G acylase and 
      beta-lactamase in Escherichia coli.
PG  - 94-101
AB  - AIMS: The present work aimed to improve the production of penicillin G acylase 
      (PGA) and reduce the beta-lactamase activity through acridine orange (AO) induced 
      mutation in Escherichia coli. METHODS AND RESULTS: Three wild E. coli strains 
      BDCS-N-FMu10, BDCS-N-S21 and BDCS-N-W50, producing both the enzymes PGA and 
      beta-lactamase were treated by AO. Minimum inhibitory concentration of AO was 10 
      microg ml(-1) and it was noted that bacterial growth was gradually suppressed by 
      increasing the concentration of AO from 10 to 100 microg ml(-1). The highest 
      concentration that gave permissible growth rate was 50 microg ml(-1). The 
      isolated survivals were screened on the bases of PGA and beta-lactamase 
      activities. Among the retained mutants, the occurrence of beta-lactamase 
      deficient ones (91%) was significantly higher than penicillin acylase deficient 
      ones (27%). CONCLUSIONS: In seven of the mutants, PGA activity was enhanced with 
      considerable decrease in beta-lactamase activity. One of the mutant strains 
      (BDCS-N-M36) exhibited very negligible expression of beta-lactamase activity and 
      twofold increase in PGA activity [12.7 mg 6-amino-penicillanic acid (6-APA) h(-1) 
      mg(-1) wet cells] compared with that in the wild-type strain (6.3 mg 6-APA h(-1) 
      mg(-1) wet cells). SIGNIFICANCE AND IMPACT OF THE STUDY: The treatment of E. coli 
      cells with AO resulted in mutants with enhanced production of PGA and 
      inactivation of beta-lactamase. These mutants could be used for industrial 
      production of PGA.
FAU - Arshad, R
AU  - Arshad R
AD  - Nuclear Institute for Agriculture and Biology (NIAB), Faisalabad, Pakistan. 
      arshadrubina@hotmail.com
FAU - Farooq, S
AU  - Farooq S
FAU - Iqbal, N
AU  - Iqbal N
FAU - Ali, S S
AU  - Ali SS
LA  - eng
PT  - Journal Article
PL  - England
TA  - Lett Appl Microbiol
JT  - Letters in applied microbiology
JID - 8510094
RN  - EC 3.5.1.11 (Penicillin Amidase)
RN  - EC 3.5.2.6 (beta-Lactamases)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange/*pharmacology
MH  - Enzyme Induction/drug effects
MH  - Escherichia coli/*drug effects/*enzymology
MH  - Gene Expression Regulation, Bacterial/*drug effects
MH  - Mutagenesis
MH  - Penicillin Amidase/genetics/*metabolism
MH  - beta-Lactamases/drug effects/*metabolism
EDAT- 2006/01/31 09:00
MHDA- 2006/05/26 09:00
CRDT- 2006/01/31 09:00
PHST- 2006/01/31 09:00 [pubmed]
PHST- 2006/05/26 09:00 [medline]
PHST- 2006/01/31 09:00 [entrez]
AID - LAM1819 [pii]
AID - 10.1111/j.1472-765X.2005.01819.x [doi]
PST - ppublish
SO  - Lett Appl Microbiol. 2006 Feb;42(2):94-101. doi: 
      10.1111/j.1472-765X.2005.01819.x.