PMID- 16443372
OWN - NLM
STAT- MEDLINE
DCOM- 20061122
LR  - 20181201
IS  - 1044-7431 (Print)
IS  - 1095-9327 (Electronic)
IS  - 1044-7431 (Linking)
VI  - 31
IP  - 4
DP  - 2006 Apr
TI  - Transforming growth factor alpha attenuates the functional expression of AMPA 
      receptors in cortical GABAergic neurons.
PG  - 628-41
AB  - In the developing neocortex, brain-derived neurotrophic factor (BDNF) exerts a 
      trophic activity to increase the expression and channel activity of 
      alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)-type glutamate 
      receptor subunits. Here, we demonstrate that the epidermal growth factor (EGF) 
      receptor (ErbB1) ligands exert the opposite biological activity in cultured 
      neocortical neurons. Subchronic stimulation of ErbB1 with transforming growth 
      factor alpha (TGFalpha), EGF, or heparin-binding EGF (HB-EGF) down-regulated 
      protein expression of the GluR1 AMPA receptor subunit in cultured neocortical 
      neurons. In agreement, TGFalpha treatment decreased the Bmax of [3H] AMPA binding 
      and GluR1 mRNA levels. Immunocytochemistry revealed that the decrease in GluR1 
      was most pronounced in multipolar GABAergic neurons. To examine the physiological 
      consequences, we recorded AMPA-evoked currents as well as miniature excitatory 
      postsynaptic currents in morphologically identified putative GABAergic neurons in 
      culture. Subchronic TGFalpha treatment decreased AMPA-triggered currents as well 
      as the amplitude and frequency of miniature excitatory postsynaptic currents. An 
      ErbB1 tyrosine kinase inhibitor, PD153035, inhibited the TGFalpha effect. 
      Moreover, TGFalpha counteracted the neurotrophic activity of BDNF on AMPA 
      receptor expression. Co-application of TGFalpha with BDNF blocked the 
      BDNF-triggered up-regulation of AMPA receptor expression and currents. These 
      observations reveal a negative regulatory activity of the ErbB1 ligand, TGFalpha, 
      which reduces the input sensitivity of cortical GABAergic neurons to attenuate 
      their inhibitory function.
FAU - Namba, Hisaaki
AU  - Namba H
AD  - Department of Molecular Neurobiology, Brain Research Institute, Niigata 
      University, 1-757 Asahimachi, Niigata 951-8585, Japan.
FAU - Nagano, Tadasato
AU  - Nagano T
FAU - Iwakura, Yuriko
AU  - Iwakura Y
FAU - Xiong, Huabao
AU  - Xiong H
FAU - Jourdi, Hussam
AU  - Jourdi H
FAU - Takei, Nobuyuki
AU  - Takei N
FAU - Nawa, Hiroyuki
AU  - Nawa H
LA  - eng
GR  - P01 NS045260/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060126
PL  - United States
TA  - Mol Cell Neurosci
JT  - Molecular and cellular neurosciences
JID - 9100095
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Culture Media, Serum-Free)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Hbegf protein, rat)
RN  - 0 (Heparin-binding EGF-like Growth Factor)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Ligands)
RN  - 0 (Protein Subunits)
RN  - 0 (Quinazolines)
RN  - 0 (Receptors, AMPA)
RN  - 0 (Transforming Growth Factor alpha)
RN  - 56-12-2 (gamma-Aminobutyric Acid)
RN  - 62229-50-9 (Epidermal Growth Factor)
RN  - 77521-29-0 (alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid)
RN  - EC 2.7.10.1 (ErbB Receptors)
RN  - TC62B68RSL (4-((3-bromophenyl)amino)-6,7-dimethoxyquinazoline)
RN  - TFZ3H25BS1 (glutamate receptor ionotropic, AMPA 1)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/metabolism
MH  - Cells, Cultured
MH  - Cerebral Cortex/*cytology/metabolism
MH  - Culture Media, Serum-Free
MH  - Electrophysiology
MH  - Enzyme Inhibitors/pharmacology
MH  - Epidermal Growth Factor/metabolism
MH  - ErbB Receptors/antagonists & inhibitors/*metabolism
MH  - Heparin-binding EGF-like Growth Factor
MH  - Intercellular Signaling Peptides and Proteins
MH  - Ligands
MH  - Neurons/cytology/drug effects/*metabolism
MH  - Protein Subunits/metabolism
MH  - Quinazolines/pharmacology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, AMPA/*metabolism
MH  - Transforming Growth Factor alpha/*metabolism/pharmacology
MH  - alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid/metabolism
MH  - gamma-Aminobutyric Acid/*metabolism
PMC - PMC3683556
MID - NIHMS469080
EDAT- 2006/01/31 09:00
MHDA- 2006/12/09 09:00
PMCR- 2013/06/16
CRDT- 2006/01/31 09:00
PHST- 2005/08/08 00:00 [received]
PHST- 2005/11/05 00:00 [revised]
PHST- 2005/12/08 00:00 [accepted]
PHST- 2006/01/31 09:00 [pubmed]
PHST- 2006/12/09 09:00 [medline]
PHST- 2006/01/31 09:00 [entrez]
PHST- 2013/06/16 00:00 [pmc-release]
AID - S1044-7431(05)00301-5 [pii]
AID - 10.1016/j.mcn.2005.12.002 [doi]
PST - ppublish
SO  - Mol Cell Neurosci. 2006 Apr;31(4):628-41. doi: 10.1016/j.mcn.2005.12.002. Epub 
      2006 Jan 26.