PMID- 16447156
OWN - NLM
STAT- MEDLINE
DCOM- 20060511
LR  - 20131121
IS  - 1615-9853 (Print)
IS  - 1615-9853 (Linking)
VI  - 6
IP  - 6
DP  - 2006 Mar
TI  - Intermittent administration of morphine alters protein expression in rat nucleus 
      accumbens.
PG  - 2003-8
AB  - Repeated exposure to drugs of abuse causes time-dependent neuroadaptive changes 
      in the mesocorticolimbic system of the brain that are considered to underlie the 
      expression of major behavioral characteristics of drug addiction. We used a 2-D 
      gel-based proteomics approach to examine morphine-induced temporal changes in 
      protein expression and/or PTM in the nucleus accumbens (NAc) of 
      morphine-sensitized rats. Rats were pretreated with saline [1 mL/kg 
      subcutaneously (s.c.)] or morphine (10 mg/kg, s.c.) once daily for 14 days and 
      the animals were decapitated 1 day later. The NAc was extracted and proteins 
      resolved by 2-DE. Several protein functional groups were found to be regulated in 
      the morphine-treated group, representing cytoskeletal proteins, proteins involved 
      in neurotransmission, enzymes involved in energy metabolism and protein 
      degradation, and a protein that regulates translation.
FAU - Li, Ka Wan
AU  - Li KW
AD  - Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and 
      Cognitive Research, Vrije Universiteit, Amsterdam, The Netherlands. 
      ka.wan.li@falw.vu.nl
FAU - Jimenez, Connie R
AU  - Jimenez CR
FAU - van der Schors, Roel C
AU  - van der Schors RC
FAU - Hornshaw, Martin P
AU  - Hornshaw MP
FAU - Schoffelmeer, Anton N M
AU  - Schoffelmeer AN
FAU - Smit, August B
AU  - Smit AB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Proteomics
JT  - Proteomics
JID - 101092707
RN  - 0 (Narcotics)
RN  - 0 (Proteins)
RN  - 76I7G6D29C (Morphine)
RN  - EC 3.4.- (Peptide Hydrolases)
SB  - IM
MH  - Animals
MH  - Drug Administration Schedule
MH  - Electrophoresis, Gel, Two-Dimensional
MH  - Injections, Subcutaneous
MH  - Isoelectric Point
MH  - Male
MH  - Mass Spectrometry
MH  - Molecular Weight
MH  - Morphine/*administration & dosage
MH  - Narcotics/*administration & dosage
MH  - Nucleus Accumbens/*metabolism
MH  - Peptide Hydrolases/metabolism
MH  - Peptide Mapping
MH  - Proteins/chemistry/*metabolism
MH  - Proteomics/*methods
MH  - Rats
MH  - Rats, Wistar
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
EDAT- 2006/02/01 09:00
MHDA- 2006/05/12 09:00
CRDT- 2006/02/01 09:00
PHST- 2006/02/01 09:00 [pubmed]
PHST- 2006/05/12 09:00 [medline]
PHST- 2006/02/01 09:00 [entrez]
AID - 10.1002/pmic.200500045 [doi]
PST - ppublish
SO  - Proteomics. 2006 Mar;6(6):2003-8. doi: 10.1002/pmic.200500045.