PMID- 16447252
OWN - NLM
STAT- MEDLINE
DCOM- 20060609
LR  - 20060403
IS  - 0021-9541 (Print)
IS  - 0021-9541 (Linking)
VI  - 207
IP  - 3
DP  - 2006 Jun
TI  - TP53 and p16INK4A, but not H-KI-Ras, are involved in tumorigenesis and 
      progression of pleomorphic adenomas.
PG  - 654-9
AB  - The putative role of TP53 and p16(INK4A) tumor suppressor genes and Ras oncogenes 
      in the development and progression of salivary gland neoplasias was studied in 28 
      cases of pleomorphic adenomas (PA), 4 cases of cystic adenocarcinomas, and 1 case 
      of carcinoma ex-PA. Genetic and epigenetic alterations in the above genes were 
      analyzed by Polymerase Chain Reaction/Single Strand Conformational Polymorphism 
      (PCR/SSCP) and sequencing and by Methylation Specific-PCR (MS-PCR). Mutations in 
      TP53 were found in 14% (4/28) of PAs and in 60% (3/5) of carcinomas. Mutations in 
      H-Ras and K-Ras were identified in 4% (1/28) and 7% (2/28) of PAs, respectively. 
      Only 20% (1/5) of carcinomas screened displayed mutations in K-Ras. p16(INK4A) 
      promoter hypermethylation was found in 14% (4/28) of PAs and 100% (5/5) 
      carcinomas. All genetic and epigenetic alterations were detected exclusively in 
      the epithelial and transitional tumor components, and were absent in the 
      mesenchymal parts. Our analysis suggests that TP53 mutations and p16(INK4A) 
      promoter methylation, but not alterations in the H-Ras and K-Ras genes, might be 
      involved in the malignant progression of PA into carcinoma.
CI  - Copyright 2006 Wiley-Liss, Inc.
FAU - Augello, Claudia
AU  - Augello C
AD  - Department of Oncology, Universita of Palermo, Palermo, Italy.
FAU - Gregorio, Valter
AU  - Gregorio V
FAU - Bazan, Viviana
AU  - Bazan V
FAU - Cammareri, Patrizia
AU  - Cammareri P
FAU - Agnese, Valentina
AU  - Agnese V
FAU - Cascio, Sandra
AU  - Cascio S
FAU - Corsale, Simona
AU  - Corsale S
FAU - Calo, Valentina
AU  - Calo V
FAU - Gullo, Arianna
AU  - Gullo A
FAU - Passantino, Rita
AU  - Passantino R
FAU - Gargano, Grazia
AU  - Gargano G
FAU - Bruno, Loredana
AU  - Bruno L
FAU - Rinaldi, Gaetana
AU  - Rinaldi G
FAU - Morello, Vincenza
AU  - Morello V
FAU - Gerbino, Aldo
AU  - Gerbino A
FAU - Tomasino, Rosa Maria
AU  - Tomasino RM
FAU - Macaluso, Marcella
AU  - Macaluso M
FAU - Surmacz, Eva
AU  - Surmacz E
FAU - Russo, Antonio
AU  - Russo A
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Cell Physiol
JT  - Journal of cellular physiology
JID - 0050222
RN  - 0 (Cyclin-Dependent Kinase Inhibitor p16)
RN  - 0 (Tumor Suppressor Protein p53)
RN  - EC 3.6.5.2 (Proto-Oncogene Proteins p21(ras))
SB  - IM
MH  - Adenoma/*genetics/metabolism/*pathology
MH  - Base Sequence
MH  - Cell Transformation, Neoplastic/*genetics/metabolism/*pathology
MH  - Cyclin-Dependent Kinase Inhibitor p16/*genetics/metabolism
MH  - Disease Progression
MH  - Epigenesis, Genetic/genetics
MH  - Genotype
MH  - Humans
MH  - Methylation
MH  - Mutation/genetics
MH  - Proto-Oncogene Proteins p21(ras)/genetics
MH  - Tumor Suppressor Protein p53/*genetics
EDAT- 2006/02/01 09:00
MHDA- 2006/06/10 09:00
CRDT- 2006/02/01 09:00
PHST- 2006/02/01 09:00 [pubmed]
PHST- 2006/06/10 09:00 [medline]
PHST- 2006/02/01 09:00 [entrez]
AID - 10.1002/jcp.20601 [doi]
PST - ppublish
SO  - J Cell Physiol. 2006 Jun;207(3):654-9. doi: 10.1002/jcp.20601.