PMID- 16447816
OWN - NLM
STAT- MEDLINE
DCOM- 20060912
LR  - 20161020
IS  - 0884-6812 (Linking)
VI  - 27
IP  - 5
DP  - 2005 Oct
TI  - Aneuploidy, centrosome alteration and securin overexpression as features of 
      pituitary somatotroph and lactotroph adenomas.
PG  - 241-52
AB  - OBJECTIVE: To verify the presence of numerical chromosomal aberrations (NCAs) in 
      different types of pituitary adenomas (PAs) and to investigate 2 of the 
      mechanisms that are possibly related to aneuploidies in PAs: securin 
      overexpression and centrosome alterations. STUDY DESIGN: Twenty-one PAs of 
      different types were analyzed with interphase fluorescence in situ hybridization 
      (FISH) on paraffin sections with centromeric probes for chromosomes 2, 3, 8, 11 
      and 12. In all cases, the immunohistochemical expression of securin was evaluated 
      and the number of cells with abnormal nuclear shape recorded. The ultrastructural 
      study of centrosomes was performed in a subset of 12 tumors. RESULTS: At 
      interphase FISH analysis, growth hormone (GH)-cell and prolactin (PRL)-cell PAs 
      showed multiple chromosome gains and a low frequency of chromosome losses, 
      suggesting a hyperdiploid chromosome assessment. In contrast, in the other types 
      of PAs a lower frequency of NCAs was observed. In addition, when compared to 
      other types of PAs, GH-cell and PRL-cell adenomas showed overexpression of 
      securin and a higher number of both cells with abnormal nuclear shape and cells 
      with centrosomes. CONCLUSION: Somatotroph and lactotroph adenomas are 
      characterized by aneuploidy, abnormal nuclear shape and centrosome amplification, 
      which are possibly related to securin overexpression.
FAU - Uccella, Silvia
AU  - Uccella S
AD  - Department of Human Morphology, University of Insubria and Ospedale di Circolo, 
      Varese, Italy. silvia.uccella@uninsubria.it
FAU - Tibiletti, Maria Grazia
AU  - Tibiletti MG
FAU - Bernasconi, Barbara
AU  - Bernasconi B
FAU - Finzi, Giovanna
AU  - Finzi G
FAU - Oldrini, Rita
AU  - Oldrini R
FAU - Capella, Carlo
AU  - Capella C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Anal Quant Cytol Histol
JT  - Analytical and quantitative cytology and histology
JID - 8506819
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Securin)
RN  - 0 (pituitary tumor-transforming protein 1, human)
SB  - IM
MH  - Adult
MH  - Aged
MH  - *Aneuploidy
MH  - Cell Nucleus/genetics/metabolism
MH  - Cell Proliferation
MH  - Centromere/genetics/*pathology
MH  - Female
MH  - Gene Expression Regulation, Neoplastic/genetics
MH  - Growth Hormone-Secreting Pituitary 
      Adenoma/*genetics/metabolism/*pathology/ultrastructure
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Microscopy, Electron
MH  - Middle Aged
MH  - Neoplasm Proteins/genetics/*metabolism
MH  - Prolactinoma/*genetics/metabolism/*pathology
MH  - Securin
EDAT- 2006/02/02 09:00
MHDA- 2006/09/13 09:00
CRDT- 2006/02/02 09:00
PHST- 2006/02/02 09:00 [pubmed]
PHST- 2006/09/13 09:00 [medline]
PHST- 2006/02/02 09:00 [entrez]
PST - ppublish
SO  - Anal Quant Cytol Histol. 2005 Oct;27(5):241-52.