PMID- 16449281
OWN - NLM
STAT- MEDLINE
DCOM- 20061122
LR  - 20151119
IS  - 0931-0509 (Print)
IS  - 0931-0509 (Linking)
VI  - 21
IP  - 6
DP  - 2006 Jun
TI  - The effect of immunosuppressive therapy on the messenger RNA expression of target 
      genes in the urinary sediment of patients with active lupus nephritis.
PG  - 1534-40
AB  - BACKGROUND: Previous studies have shown that messenger RNA (mRNA) expression of 
      target genes is increased in the urinary sediment of patients with active lupus. 
      We study the effect of immunosuppressive therapy on the urinary gene expression 
      profile in patients with active lupus nephritis. Method. We recruited nine 
      patients with active systemic lupus erythematosus (SLE) and renal disease, and 
      required corticosteroid, with or without cytotoxic treatment. They were followed 
      for 6 months, urine samples were collected at 0, 4, 12 and 24 weeks and gene 
      expression profile was determined by polymerase chain reactions. The pattern of 
      gene expression was compared to clinical parameters of therapeutic response. 
      RESULTS: Amongst the target genes studied, there was a progressive decline in the 
      urinary expression of T-bet, interleukin (IL)-10, transforming growth factor-beta 
      (TGF-beta), monocyte chemoattractant protein-1 (MCP-1), and interferon-gamma 
      (IFN-gamma) after immunosuppressive treatment, although the change of IFN-gamma 
      was not statistically significant. The time course of their urinary expression 
      was parallel to the systemic activity as reflected by the systemic lupus 
      erythematosus disease activity index (SLEDAI). Throughout the study period, the 
      SLEDAI score correlated significantly with the expressions of IFN-gamma (r = 
      0.43, P = 0.009), T-bet (r = 0.40, P = 0.016), TGF-beta (r = 0.51, P = 0.002) and 
      MCP-1 (r = 0.38, P = 0.022). The anti-double strand(anti-ds)DNA antibody titer 
      correlated significantly with the expressions of IFN-gamma (r = 0.45, P = 0.009), 
      T-bet (r = 0.37, P = 0.034), IL-10 (r = 0.59, P<0.001), TGF-beta (r = 0.44, P = 
      0.010) and MCP-1 (r = 0.49, P = 0.004). On the other hand, the expression level 
      of IL-2, IL-4, IL-12, IL-18 and GATA-3 remained static throughout the study 
      period. CONCLUSIONS: The mRNA expression of T-bet, IL-10, TGF-beta, MCP-1, and 
      probably IFN-gamma in the urinary sediment of patients with active lupus 
      nephritis improves with successful immunosuppressive therapy, and the change in 
      gene expression profile is in phase with the clinical disease activity. 
      Measurement of urinary mRNA expression of target genes may be a potential 
      non-invasive tool for the monitoring of lupus disease activity.
FAU - Chan, Rebecca Wing-Yan
AU  - Chan RW
AD  - Department of Medicine and Therapeutics, Prince of Wales Hospital, The Chinese 
      University of Hong Kong, Shatin, NT, China.
FAU - Lai, Fernand Mac-Moune
AU  - Lai FM
FAU - Li, Edmund Kwok-Ming
AU  - Li EK
FAU - Tam, Lai-Shan
AU  - Tam LS
FAU - Chow, Kai-Ming
AU  - Chow KM
FAU - Li, Philip Kam-Tao
AU  - Li PK
FAU - Szeto, Cheuk-Chun
AU  - Szeto CC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060131
PL  - England
TA  - Nephrol Dial Transplant
JT  - Nephrology, dialysis, transplantation : official publication of the European 
      Dialysis and Transplant Association - European Renal Association
JID - 8706402
RN  - 0 (Biomarkers)
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Immunosuppressive Agents)
RN  - 0 (RNA, Messenger)
RN  - 0 (T-Box Domain Proteins)
RN  - 0 (T-box transcription factor TBX21)
RN  - 0 (Transforming Growth Factor beta)
RN  - 130068-27-8 (Interleukin-10)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Adult
MH  - Biomarkers/urine
MH  - Chemokine CCL2/urine
MH  - Drug Monitoring/*methods
MH  - Female
MH  - Gene Expression Profiling
MH  - Gene Expression Regulation/*drug effects
MH  - Humans
MH  - Immunosuppressive Agents/*pharmacology/therapeutic use
MH  - Interferon-gamma/urine
MH  - Interleukin-10/urine
MH  - Lupus Nephritis/diagnosis/*drug therapy/*urine
MH  - Middle Aged
MH  - RNA, Messenger/analysis/*drug effects
MH  - T-Box Domain Proteins/urine
MH  - Transforming Growth Factor beta/urine
EDAT- 2006/02/02 09:00
MHDA- 2006/12/09 09:00
CRDT- 2006/02/02 09:00
PHST- 2006/02/02 09:00 [pubmed]
PHST- 2006/12/09 09:00 [medline]
PHST- 2006/02/02 09:00 [entrez]
AID - gfk102 [pii]
AID - 10.1093/ndt/gfk102 [doi]
PST - ppublish
SO  - Nephrol Dial Transplant. 2006 Jun;21(6):1534-40. doi: 10.1093/ndt/gfk102. Epub 
      2006 Jan 31.