PMID- 16450588
OWN - NLM
STAT- MEDLINE
DCOM- 20090303
LR  - 20231213
IS  - 0379-4172 (Print)
IS  - 0379-4172 (Linking)
VI  - 33
IP  - 1
DP  - 2006 Jan
TI  - Expression of human BMP-2 gene in different tissues of tobacco plants.
PG  - 56-62
AB  - The bone morphogenetic proteins (BMPs) are a family of growth factors that 
      regulate the development of bone. BMP-2 is the most effective in the induction of 
      bone tissue. A large amount of BMP-2 is needed for both bone tissue engineering 
      research and clinical application. Thus, an effective way is necessary to produce 
      sufficient BMP-2 protein. With the advance in plant biotechnology, transgenic 
      plants have been targeted as a bioreactor to produce desired recombinant 
      proteins. Here, the expression of recombinant human bmp-2 gene (rhbmp-2) was 
      studied in tobacco plants using gus as a reporter gene. The difference of 
      expression levels in root, stem and leaf tissues was analyzed by GUS activity 
      assay, semi-quantitive RT-PCR and western blotting.The results indicated that the 
      expression levels of fusion protein in root and stem tissues were significantly 
      higher than those in leaf tissue. For the protein compositions in root and stem 
      tissues were simpler than those in leaf tissue,this suggested that the 
      purification process with root and stem tissues would potentially be easier.
FAU - Gao, Yuan
AU  - Gao Y
AD  - Center of Molecular Developmental Biology, Institute of Genetics and 
      Developmental Biology, Chinese Academy of Sciences, Beijing 100080, China.
FAU - Suo, Guang-Li
AU  - Suo GL
FAU - Han, Jin
AU  - Han J
FAU - He, Zheng-Quan
AU  - He ZQ
FAU - Yao, Wei
AU  - Yao W
FAU - Dai, Jian-Wu
AU  - Dai JW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Yi Chuan Xue Bao
JT  - Yi chuan xue bao = Acta genetica Sinica
JID - 7900784
RN  - 0 (BMP2 protein, human)
RN  - 0 (Bone Morphogenetic Protein 2)
RN  - EC 3.2.1.31 (Glucuronidase)
SB  - IM
MH  - Blotting, Western
MH  - Bone Morphogenetic Protein 2/*genetics/metabolism
MH  - *Gene Expression Profiling
MH  - Glucuronidase/genetics/metabolism
MH  - Plant Leaves/genetics/metabolism
MH  - Plant Roots/genetics/metabolism
MH  - Plant Stems/genetics/metabolism
MH  - Plants, Genetically Modified/*genetics/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Nicotiana/*genetics/metabolism
EDAT- 2006/02/03 09:00
MHDA- 2009/03/04 09:00
CRDT- 2006/02/03 09:00
PHST- 2006/02/03 09:00 [pubmed]
PHST- 2009/03/04 09:00 [medline]
PHST- 2006/02/03 09:00 [entrez]
AID - S0379-4172(06)60009-7 [pii]
AID - 10.1016/S0379-4172(06)60009-7 [doi]
PST - ppublish
SO  - Yi Chuan Xue Bao. 2006 Jan;33(1):56-62. doi: 10.1016/S0379-4172(06)60009-7.