PMID- 16450788
OWN - NLM
STAT- MEDLINE
DCOM- 20060406
LR  - 20161020
IS  - 0884-6812 (Linking)
VI  - 27
IP  - 6
DP  - 2005 Dec
TI  - A systematic approach to identifying urothelial cells likely to be polysomic by 
      fluorescence in situ hybridization.
PG  - 317-22
AB  - OBJECTIVE: To determine which nuclear morphologic and background features are 
      most associated with normal and abnormal chromosome patterns in urine cells 
      evaluated by fluorescence in situ hybridization (FISH) with Vysis UroVysion 
      (Downers Grove, Illinois, USA). STUDY DESIGN: One hundred specimens were analyzed 
      and the nuclear morphologic and background features compared between 
      FISH-negative (disomic) and -positive (polysomic) cases. RESULTS: Our data show 
      that polysomic urothelial cells have significantly (p < 0.001) weaker DAPI 
      nuclear counterstaining, nuclear enlargement, more irregular nuclear shape and 
      more chromatin clumping when compared to disomic cells. CONCLUSION: These 
      findings indicate that there are specific staining and nuclear cytologic features 
      that can help identify cells that have a high probability of being polysomic by 
      FISH. This information may help cytologists decrease the time required to 
      evaluate urine specimens by FISH and may aid in the development of instruments 
      that automate FISH analysis.
FAU - Kipp, Benjamin R
AU  - Kipp BR
AD  - Molecular Cytology and Imaging Laboratory, Department of Laboratory Medicine and 
      Pathology, Mayo Clinic Foundation, Roschester, Minnesota, USA.
FAU - Fritcher, Emily G Barr
AU  - Fritcher EG
FAU - del Rosario, Kristina M
AU  - del Rosario KM
FAU - Stevens, Carole L
AU  - Stevens CL
FAU - Sebo, Thomas J
AU  - Sebo TJ
FAU - Halling, Kevin C
AU  - Halling KC
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Anal Quant Cytol Histol
JT  - Analytical and quantitative cytology and histology
JID - 8506819
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - *Aneuploidy
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Male
MH  - Middle Aged
MH  - Urologic Neoplasms/*genetics/*pathology
MH  - Urothelium/*pathology
EDAT- 2006/02/03 09:00
MHDA- 2006/04/07 09:00
CRDT- 2006/02/03 09:00
PHST- 2006/02/03 09:00 [pubmed]
PHST- 2006/04/07 09:00 [medline]
PHST- 2006/02/03 09:00 [entrez]
PST - ppublish
SO  - Anal Quant Cytol Histol. 2005 Dec;27(6):317-22.