PMID- 16453138
OWN - NLM
STAT- MEDLINE
DCOM- 20070425
LR  - 20160512
IS  - 1618-2642 (Print)
IS  - 1618-2642 (Linking)
VI  - 384
IP  - 5
DP  - 2006 Mar
TI  - Determination of fluoroquinolones in eggs using in-tube solid-phase 
      microextraction coupled to high-performance liquid chromatography.
PG  - 1228-35
AB  - A simple, rapid, and sensitive method using in-tube solid-phase microextraction 
      (in-tube SPME) based on poly(methacrylic acid-ethylene glycol dimethacrylate) 
      (MAA-EGDMA) monolith coupled to HPLC with fluorescence and UV detection was 
      developed for the determination of five fluoroquinolones (FQs). Ofloxacin (OFL), 
      norfloxacin (NOR), ciprofloxacin (CIP), enrofloxacin (ENRO), and sarafloxacin 
      (SARA) can be enriched and determined in the spiked eggs and albumins. CIP/ENRO 
      in eggs and albumins of ENRO-treated hens were also studied using the proposed 
      method. Only homogenization, dilution, and centrifugation were required before 
      the sample was supplied to the in-tube microextraction, and no organic solvents 
      were consumed in the procedures. Under the optimized extraction conditions, good 
      extraction efficiency for the five FQs was obtained with no matrix interference 
      in the process of extraction and the subsequent chromatographic separation. The 
      detection limits (S/N=3) were found to be 0.1-2.6 ng g(-1) and 0.2-2.4 ng g(-1) 
      in whole egg and egg albumin, respectively. Good linearity could be achieved over 
      the range 2-500 ng mL(-1) for the five FQs with regression coefficients above 
      0.9995 in both whole egg and albumin. The reproducibility of the method was 
      evaluated at three concentration levels, with the resulting relative standard 
      deviations (RSDs) less than 7%. The method was successfully applied to the 
      analysis of ENRO and its primary metabolite CIP in the eggs and albumins of 
      ENRO-treated hens.
FAU - Huang, Jing-Fang
AU  - Huang JF
AD  - Department of Chemistry, Wuhan University, Wuhan 430072, People's Republic of 
      China.
FAU - Lin, Bo
AU  - Lin B
FAU - Yu, Qiong-Wei
AU  - Yu QW
FAU - Feng, Yu-Qi
AU  - Feng YQ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060202
PL  - Germany
TA  - Anal Bioanal Chem
JT  - Analytical and bioanalytical chemistry
JID - 101134327
RN  - 0 (Albumins)
RN  - 0 (Ethylene Glycols)
RN  - 0 (Fluoroquinolones)
RN  - 0 (Methacrylates)
RN  - 0 (poly(methacrylic acid-ethylene glycol dimethacrylate))
SB  - IM
MH  - Albumins/chemistry
MH  - Chromatography, High Pressure Liquid/instrumentation/methods
MH  - Ethylene Glycols/chemistry
MH  - Fluoroquinolones/*analysis
MH  - Hydrogen-Ion Concentration
MH  - Methacrylates/chemistry
MH  - Molecular Structure
MH  - Ovum/*chemistry
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Solid Phase Microextraction/*methods
MH  - Spectrometry, Fluorescence/instrumentation/methods
MH  - Spectrophotometry, Ultraviolet/instrumentation/methods
MH  - Time Factors
EDAT- 2006/02/03 09:00
MHDA- 2007/04/26 09:00
CRDT- 2006/02/03 09:00
PHST- 2005/10/30 00:00 [received]
PHST- 2005/12/02 00:00 [accepted]
PHST- 2005/11/30 00:00 [revised]
PHST- 2006/02/03 09:00 [pubmed]
PHST- 2007/04/26 09:00 [medline]
PHST- 2006/02/03 09:00 [entrez]
AID - 10.1007/s00216-005-0270-8 [doi]
PST - ppublish
SO  - Anal Bioanal Chem. 2006 Mar;384(5):1228-35. doi: 10.1007/s00216-005-0270-8. Epub 
      2006 Feb 2.