PMID- 1645326
OWN - NLM
STAT- MEDLINE
DCOM- 19910702
LR  - 20181113
IS  - 0019-2805 (Print)
IS  - 1365-2567 (Electronic)
IS  - 0019-2805 (Linking)
VI  - 72
IP  - 4
DP  - 1991 Apr
TI  - Human immunoglobulin preparation for intravenous use induces elevation of 
      cellular cyclic adenosine 3':5'-monophosphate levels, resulting in suppression of 
      tumour necrosis factor alpha and interleukin-1 production.
PG  - 497-501
AB  - We previously showed that human immunoglobulin preparation for intravenous use 
      (IGIV) suppresses the in vitro production of tumour necrosis factor-alpha 
      (TNF-alpha) and interleukin-1 (IL-1) by rabbit peritoneal exudate cells (PEC) 
      stimulated with lipopolysaccharide (LPS). In this study we investigated the 
      mechanism of the suppression. IGIV treated at pH4 (pH4-G) was used as IGIV. Fc 
      fragments of pH4-G, as well as untreated pH4-G, suppressed TNF-alpha and IL-1 
      production by rabbit PEC stimulated with LPS. The interaction of pH4-G with PEC 
      also resulted in generation of cyclic adenosine 3':5'-monophosphate (cAMP), known 
      to be an intracellular second messenger. N6, 2'-0-dibutyryl cAMP (BtcAMP), a 
      lipid-soluble derivative of cAMP, and cholera toxin (CT), an adenylate cyclase 
      activating agent, also suppressed the production of TNF-alpha and IL-1. Further 
      N-[2-(methylamino) ethyl]-5-isoquinolinesulphonamide dihydrochloride (H-8), an 
      inhibitor of cAMP-dependent protein kinases, abrogated the suppression by pH4-G 
      of the productions. These results indicate that the binding of IGIV to PEC via Fc 
      gamma receptors (Fc gamma R) induces the elevation of intracellular cAMP levels, 
      resulting in the suppression of LPS-induced TNF-alpha and IL-1 productions.
FAU - Shimozato, T
AU  - Shimozato T
AD  - Biological Research Laboratories, Sankyo Co. Ltd., Tokyo, Japan.
FAU - Iwata, M
AU  - Iwata M
FAU - Kawada, H
AU  - Kawada H
FAU - Tamura, N
AU  - Tamura N
LA  - eng
PT  - Journal Article
PL  - England
TA  - Immunology
JT  - Immunology
JID - 0374672
RN  - 0 (Immunoglobulin Fab Fragments)
RN  - 0 (Immunoglobulin Fc Fragments)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Interleukin-1)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - E0399OZS9N (Cyclic AMP)
SB  - IM
MH  - Animals
MH  - Ascitic Fluid/immunology
MH  - Cyclic AMP/*metabolism
MH  - Hot Temperature
MH  - Humans
MH  - Hydrogen-Ion Concentration
MH  - *Immunization, Passive
MH  - Immunoglobulin Fab Fragments/immunology
MH  - Immunoglobulin Fc Fragments/immunology
MH  - Immunoglobulin G/immunology
MH  - Interleukin-1/*biosynthesis
MH  - Lipopolysaccharides/immunology
MH  - Macrophages/immunology
MH  - Male
MH  - Rabbits
MH  - Tumor Necrosis Factor-alpha/*biosynthesis
PMC - PMC1384367
EDAT- 1991/04/01 00:00
MHDA- 1991/04/01 00:01
PMCR- 1992/04/01
CRDT- 1991/04/01 00:00
PHST- 1991/04/01 00:00 [pubmed]
PHST- 1991/04/01 00:01 [medline]
PHST- 1991/04/01 00:00 [entrez]
PHST- 1992/04/01 00:00 [pmc-release]
PST - ppublish
SO  - Immunology. 1991 Apr;72(4):497-501.