PMID- 16453311
OWN - NLM
STAT- MEDLINE
DCOM- 20060517
LR  - 20131121
IS  - 0360-4012 (Print)
IS  - 0360-4012 (Linking)
VI  - 83
IP  - 4
DP  - 2006 Mar
TI  - Chronic morphine treatment alters expression of N-methyl-D-aspartate receptor 
      subunits in the extended amygdala.
PG  - 532-7
AB  - The nucleus accumbens (NAcc) and central amygdala (CeA) are parts of the extended 
      amygdala, a complex that plays a key role in drug abuse and dependence. Our 
      previous studies showed that opiates and ethanol alter glutamatergic transmission 
      in these regions. N-methyl-D-aspartate (NMDA) receptors are key components of 
      glutamatergic transmission likely involved in the development of opiate tolerance 
      and dependence. In this study we examined the effects of chronic morphine 
      administration on gene and protein expression of three major NMDA receptors 
      subunits (NR1, NR2A, and NR2B) in NAcc and CeA. Real-time PCR showed no 
      differences in mRNA levels of any of the subunits in the whole NAcc between naive 
      and morphine-dependent rats. However, at the protein level, immunoblotting 
      revealed that chronic morphine significantly increased levels of NR1 and NR2B 
      subunits. In contrast to the case for NAcc, in CeA we found an increased mRNA 
      level for the NR1 subunit only but unchanged protein levels of all three subunits 
      in morphine-dependent rats. The altered expressions of NMDA receptor subunits, 
      especially in NAcc, of morphine-dependent rats may represent a neuroadaptation to 
      chronic morphine and suggest a mechanism for the changes of glutamatergic 
      transmission found in the extended amygdala in dependent rats. In addition, our 
      results indicate a region-specific response of NMDA receptor subunits to chronic 
      morphine administration at the gene and protein levels.
CI  - Copyright 2006 Wiley-Liss, Inc.
FAU - Bajo, Michal
AU  - Bajo M
AD  - Molecular and Integrative Neurosciences Department, The Scripps Research 
      Institute, La Jolla, California 92037, USA.
FAU - Crawford, Elena F
AU  - Crawford EF
FAU - Roberto, Marisa
AU  - Roberto M
FAU - Madamba, Samuel G
AU  - Madamba SG
FAU - Siggins, George Robert
AU  - Siggins GR
LA  - eng
GR  - DA03665/DA/NIDA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - J Neurosci Res
JT  - Journal of neuroscience research
JID - 7600111
RN  - 0 (Actins)
RN  - 0 (NR1 NMDA receptor)
RN  - 0 (NR2A NMDA receptor)
RN  - 0 (NR2B NMDA receptor)
RN  - 0 (Narcotics)
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
RN  - 76I7G6D29C (Morphine)
SB  - IM
MH  - Actins/biosynthesis
MH  - Amygdala/drug effects/*metabolism
MH  - Animals
MH  - Blotting, Western
MH  - Gene Expression/drug effects
MH  - Male
MH  - Morphine/*pharmacology
MH  - Morphine Dependence/metabolism
MH  - Narcotics/*pharmacology
MH  - Nucleus Accumbens/drug effects/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, N-Methyl-D-Aspartate/*biosynthesis/drug effects/genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 2006/02/03 09:00
MHDA- 2006/05/18 09:00
CRDT- 2006/02/03 09:00
PHST- 2006/02/03 09:00 [pubmed]
PHST- 2006/05/18 09:00 [medline]
PHST- 2006/02/03 09:00 [entrez]
AID - 10.1002/jnr.20756 [doi]
PST - ppublish
SO  - J Neurosci Res. 2006 Mar;83(4):532-7. doi: 10.1002/jnr.20756.