PMID- 16455969
OWN - NLM
STAT- MEDLINE
DCOM- 20060314
LR  - 20210102
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 176
IP  - 4
DP  - 2006 Feb 15
TI  - Hyperthermia enhances CTL cross-priming.
PG  - 2134-41
AB  - Dendritic cells (DCs) loaded with killed allogeneic melanoma cells can 
      cross-prime naive CD8(+) T cells to differentiate into melanoma-specific CTLs in 
      3-wk cultures. In this study we show that DCs loaded with killed melanoma cells 
      that were heated to 42 degrees C before killing are more efficient in 
      cross-priming of naive CD8(+) T cells than DCs loaded with unheated killed 
      melanoma cells. The enhanced cross-priming was demonstrated by several 
      parameters: 1) induction of naive CD8(+) T cell differentiation in 2-wk cultures, 
      2) enhanced killing of melanoma peptide-pulsed T2 cells, 3) enhanced killing of 
      HLA-A*0201(+) melanoma cells in a standard 4-h chromium release assay, and 4) 
      enhanced capacity to prevent tumor growth in vitro in a tumor regression assay. 
      Two mechanisms might explain the hyperthermia-induced enhanced cross-priming. 
      First, heat-treated melanoma cells expressed increased levels of 70-kDa heat 
      shock protein (HSP70), and enhanced cross-priming could be reproduced by 
      overexpression of HSP70 in melanoma cells transduced with HSP70 encoding 
      lentiviral vector. Second, hyperthermia resulted in the increased transcription 
      of several tumor Ag-associated Ags, including MAGE-B3, -B4, -A8, and -A10. Thus, 
      heat treatment of tumor cells permits enhanced cross-priming, possibly via 
      up-regulation of both HSPs and tumor Ag expression.
FAU - Shi, Hongzhen
AU  - Shi H
AD  - Baylor Institute for Immunology Research, Dallas, TX 75204, USA.
FAU - Cao, Tinghua
AU  - Cao T
FAU - Connolly, John E
AU  - Connolly JE
FAU - Monnet, Laurence
AU  - Monnet L
FAU - Bennett, Lynda
AU  - Bennett L
FAU - Chapel, Sylvie
AU  - Chapel S
FAU - Bagnis, Claude
AU  - Bagnis C
FAU - Mannoni, Patrice
AU  - Mannoni P
FAU - Davoust, Jean
AU  - Davoust J
FAU - Palucka, A Karolina
AU  - Palucka AK
FAU - Banchereau, Jacques
AU  - Banchereau J
LA  - eng
GR  - CA085540/CA/NCI NIH HHS/United States
GR  - CA78846/CA/NCI NIH HHS/United States
GR  - CA89440/CA/NCI NIH HHS/United States
GR  - P0-1CA84512/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Antigens, Neoplasm)
RN  - 0 (HSP70 Heat-Shock Proteins)
RN  - 0 (Histocompatibility Antigens Class I)
SB  - IM
MH  - Amino Acid Sequence
MH  - Antigens, Neoplasm/metabolism
MH  - Apoptosis
MH  - Cell Line, Tumor
MH  - Cross-Priming/*immunology
MH  - Dendritic Cells/immunology/metabolism
MH  - Gene Expression Regulation
MH  - HSP70 Heat-Shock Proteins/chemistry/metabolism
MH  - Histocompatibility Antigens Class I/metabolism
MH  - Humans
MH  - *Hyperthermia, Induced
MH  - Melanoma/metabolism/pathology
MH  - Molecular Sequence Data
MH  - T-Lymphocytes, Cytotoxic/chemistry/*immunology/metabolism
MH  - Temperature
EDAT- 2006/02/04 09:00
MHDA- 2006/03/15 09:00
CRDT- 2006/02/04 09:00
PHST- 2006/02/04 09:00 [pubmed]
PHST- 2006/03/15 09:00 [medline]
PHST- 2006/02/04 09:00 [entrez]
AID - 176/4/2134 [pii]
AID - 10.4049/jimmunol.176.4.2134 [doi]
PST - ppublish
SO  - J Immunol. 2006 Feb 15;176(4):2134-41. doi: 10.4049/jimmunol.176.4.2134.