PMID- 16456776
OWN - NLM
STAT- MEDLINE
DCOM- 20060410
LR  - 20061115
IS  - 1003-9406 (Print)
IS  - 1003-9406 (Linking)
VI  - 23
IP  - 1
DP  - 2006 Feb
TI  - Average-12.9 chromosome imbalances coupling with 15 differential expression genes 
      possibly involved in the carcinogenesis, progression and metastasis of 
      supraglottic laryngeal squamous cell cancer.
PG  - 7-11
AB  - OBJECTIVE: With the objective of discovering novel putative chromosomal regions 
      and special genes involved in the carcinogenesis, progression and metastasis of 
      laryngeal squamous cell cancer (LSCC). METHODS: DNA copy profile of LSCC were 
      obtained and analyzed by comparative genomic hybridization (CGH) and a 
      computerized digital image analysis system. cDNA microarray of LSCC was performed 
      and the profile was analyzed by Hierarchical clustering. RESULTS: CGH analysis 
      showed average-12.9 gains and losses of chromosomes in LSCC. Relatively high 
      frequencies of gains were found at 3q15-21 (14/18), 5p12-13 (11/18), 8q22-24 
      (6/18), 11q12-13 (8/18), 15q21-23 (7/18) and 18p11 (8/18), while those of losses 
      at 1p13-21 (8/18), 3p21-23 (14/18), 5q21-22 (14/18), 9p12-pter (11/18) and 
      13q21-31 (8/18). Hierarchical clustering analysis showed that the differentially 
      expressed genes were segregated into three groups. Three genes differentially 
      expressed in process I (normal tissue to cancer) and process II (cancer to lymph 
      node metastasis), and the Cy5/Cy3 ratios of twelve genes were either higher than 
      5.0 or lower than 0.2 in process I or process II. The fifteen special genes were 
      first reported possibly to be the relationships with LSCC. In particular, 4 genes 
      of them, which were cytochrome C oxidase Va, PPBP, EPHX2 and PON1, were first 
      reported to correlate with tumorigenesis. SH3GL2, which was one of the 15 special 
      genes, was located at one of the special chromosome regions, 9p12-pter. 
      CONCLUSION: The important genes and special chromosomal aberrances might provide 
      us a clue for further investigation of carcinogenesis, progression and metastasis 
      in LSCC.
FAU - Fu, Wei-neng
AU  - Fu WN
AD  - Department of Medical Genetics, China Medical University, Shenyang, Liaoning 
      110001, P.R.China.
FAU - Shang, Chao
AU  - Shang C
FAU - Huang, Dai-fa
AU  - Huang DF
FAU - Xu, Zhen-ming
AU  - Xu ZM
FAU - Sun, Xing-he
AU  - Sun XH
FAU - Sun, Kai-lai
AU  - Sun KL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi
JT  - Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese 
      journal of medical genetics
JID - 9425197
RN  - 0 (DNA, Neoplasm)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Carcinoma, Squamous Cell/*genetics/pathology
MH  - *Chromosome Aberrations
MH  - DNA, Neoplasm/analysis
MH  - Disease Progression
MH  - Female
MH  - Gene Expression
MH  - Humans
MH  - *Karyotyping
MH  - Laryngeal Neoplasms/*genetics/pathology
MH  - Male
MH  - Middle Aged
MH  - Neoplasm Metastasis
MH  - Nucleic Acid Hybridization
MH  - Oligonucleotide Array Sequence Analysis
EDAT- 2006/02/04 09:00
MHDA- 2006/04/11 09:00
CRDT- 2006/02/04 09:00
PHST- 2006/02/04 09:00 [pubmed]
PHST- 2006/04/11 09:00 [medline]
PHST- 2006/02/04 09:00 [entrez]
AID - 940623002 [pii]
PST - ppublish
SO  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2006 Feb;23(1):7-11.