PMID- 1645714
OWN - NLM
STAT- MEDLINE
DCOM- 19910710
LR  - 20210210
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 266
IP  - 17
DP  - 1991 Jun 15
TI  - Disassociation of the macrophage-maturational effects of vitamin D from 
      respiratory burst priming.
PG  - 10888-92
AB  - During the process of enhancing monocytic differentiation of the human leukemia 
      line HL-60, 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) also "primes" the cell for 
      respiratory burst by increasing the uptake of Ca2+ across the plasma membrane 
      (Hruska, K.A., Bar-Shavit, Z., Malone, J.D., and Teitelbaum, S.L. (1988) J. Biol. 
      Chem. 263, 16039-16044). The present study asked if the maturational effect of 
      vitamin D is dependent upon this "priming" phenomenon. To this end, we exposed 
      HL-60 to either 1,25(OH)2D3 or its synthetic analogue (1 alpha, 3 beta, 5Z, 
      7E)-9-10-Secocholesta-5,7,10(19)-triene-1, 3, 25-triol (22-oxa). We found that 
      22-oxa induced HL-60 maturation as effectively as does the natural steroid. As 
      expected, 48 h of 1,25(OH)2D3 exposure more than doubles (p less than 0.005) 
      HL-60 basal cytosolic Ca2+ and increases inositol triphosphate-sensitive Ca2+ 
      stores approximately 4-fold (p less than 0.01). 22-oxa in contrast alters neither 
      Ca(2+)- nor inositol triphosphate-mobilizable deposits. Moreover, 1,25(OH)2D3 
      treatment prompts a transient Ca2+ "spike" in response to 
      formyl-methionyl-leucyl-phenylalanine (fMLP) and a marked increase in superoxide 
      (O-2) generation when exposed to the chemotactic peptide (p less than 0.01) or 
      phorbol ester (p less than 0.02). Treatment with 22-oxa does not enable HL-60 to 
      respond to fMLP with a Ca2+ spike or prime the cell for respiratory burst unless 
      it is co-incubated with the Ca2+ ionophore, ionomycin. Similarly, phorbol ester 
      impacts more profoundly on O-2 generation by 1,25(OH)2D3 than 22-oxa preincubated 
      cells (p less than 0.02), unless the latter is added with ionomycin. Our findings 
      indicate that the maturational effects of vitamin D sterols are independent of 
      their capacity to prime cells for respiratory burst and that the Ca2+ 
      ionophoretic effects of 1,25(OH)2D3 play a major role in such priming.
FAU - Tanaka, H
AU  - Tanaka H
AD  - Department of Pathology, Jewish Hospital, Washington University Medical Center, 
      St. Louis, Missouri 63110.
FAU - Hruska, K A
AU  - Hruska KA
FAU - Seino, Y
AU  - Seino Y
FAU - Malone, J D
AU  - Malone JD
FAU - Nishii, Y
AU  - Nishii Y
FAU - Teitelbaum, S L
AU  - Teitelbaum SL
LA  - eng
GR  - AR32087/AR/NIAMS NIH HHS/United States
GR  - DE05413/DE/NIDCR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Antineoplastic Agents)
RN  - 0 (Receptors, Calcitriol)
RN  - 0 (Receptors, Formyl Peptide)
RN  - 0 (Receptors, Immunologic)
RN  - 0 (Receptors, Steroid)
RN  - 11062-77-4 (Superoxides)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - FXC9231JVH (Calcitriol)
RN  - N2UJM5NBF6 (maxacalcitol)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Antineoplastic Agents/pharmacology
MH  - Calcitriol/*analogs & derivatives/metabolism/*pharmacology
MH  - Calcium/*metabolism
MH  - Cell Differentiation/*drug effects
MH  - Cell Line
MH  - Cytoplasm/metabolism
MH  - Humans
MH  - Leukemia, Promyelocytic, Acute
MH  - Macrophages/*cytology/drug effects
MH  - N-Formylmethionine Leucyl-Phenylalanine/pharmacology
MH  - Receptors, Calcitriol
MH  - Receptors, Formyl Peptide
MH  - Receptors, Immunologic/drug effects/metabolism
MH  - Receptors, Steroid/drug effects/*metabolism
MH  - Superoxides/*metabolism
MH  - Tetradecanoylphorbol Acetate/pharmacology
EDAT- 1991/06/15 00:00
MHDA- 1991/06/15 00:01
CRDT- 1991/06/15 00:00
PHST- 1991/06/15 00:00 [pubmed]
PHST- 1991/06/15 00:01 [medline]
PHST- 1991/06/15 00:00 [entrez]
AID - S0021-9258(18)99102-7 [pii]
PST - ppublish
SO  - J Biol Chem. 1991 Jun 15;266(17):10888-92.