PMID- 16457698
OWN - NLM
STAT- MEDLINE
DCOM- 20060227
LR  - 20181113
IS  - 1465-542X (Electronic)
IS  - 1465-5411 (Print)
IS  - 1465-5411 (Linking)
VI  - 7
IP  - 6
DP  - 2005
TI  - Prognostic relevance of disseminated tumor cells in the bone marrow and 
      biological factors of 265 primary breast carcinomas.
PG  - R1174-85
AB  - INTRODUCTION: The prognostic significance of disseminated tumor cells in the bone 
      marrow (DTC-BM) of breast cancer patients has been demonstrated in many studies. 
      Yet, it is not clear which of the primary tumors' biological factors predict 
      hematogenous dissemination. We therefore examined 'tissue micro arrays' (TMAs) of 
      265 primary breast carcinomas from patients with known bone marrow (BM) status 
      for HER2, Topoisomerase IIalpha (Top IIa), Ki 67, and p53. METHODS: BM analysis 
      was performed by cytospin preparation and immunocytochemical staining for 
      cytokeratin (CK). TMAs were examined by immunohistochemistry (IHC) for HER2, Top 
      IIa, Ki 67 and p53, and fluorescence in situ hybridization (FISH) for HER2. 
      RESULTS: HER2 (2+/3+) was positive in 35/167 (21%) cases (FISH 24.3%), Top IIa 
      (>10%) in 87/187 (46%), Ki 67 in 52/184 (28%) and p53 (>5%) in 61/174 cases 
      (34%). Of 265 patients, 68 (25.7%) showed DTC-BM with a median of 2/2 x 106 cells 
      (1 to 1,500). None of the examined factors significantly predicted BM positivity. 
      Significant correlation was seen between HER2 IHC and Top IIa (p = 0.06), Ki 67 
      (p = 0.031), and p53 (p < .001). Top IIa correlated with Ki 67 and p53, and Ki 67 
      also with p53 (p = 0.004). After a median follow-up of 60.5 months (7 to 255), 
      the presence of DTC-BM showed prognostic relevance for overall survival (p = 
      0.03), whereas HER2 (IHC, p = 0.04; FISH, p = 0.03) and Ki 67 (p = 0.04) 
      correlated with disease free survival, and HER2 with distant disease free 
      survival (IHC, p = 0.06; FISH, p = 0.05). DISCUSSION: The congruence of the 
      examined factors' expression rates indicates a causal line of suppressor, 
      proliferation, and mitosis markers, and growth factor receptors. Hematogenous 
      tumor cell spread seems to be an independent process. The examination of these 
      factors on DTC-BM is the aim of ongoing research.
FAU - Schindlbeck, Christian
AU  - Schindlbeck C
AD  - 1st Department of Obstetrics and Gynecology Klinikum, 
      Ludwig-Maximilians-University, D-80337 Munich, Germany. 
      Christian.Schindlbeck@med.uni-muenchen.de
FAU - Kampik, Theresa
AU  - Kampik T
FAU - Janni, Wolfgang
AU  - Janni W
FAU - Rack, Brigitte
AU  - Rack B
FAU - Jeschke, Udo
AU  - Jeschke U
FAU - Krajewski, Stan
AU  - Krajewski S
FAU - Sommer, Harald
AU  - Sommer H
FAU - Friese, Klaus
AU  - Friese K
LA  - eng
PT  - Journal Article
DEP - 20051124
PL  - England
TA  - Breast Cancer Res
JT  - Breast cancer research : BCR
JID - 100927353
RN  - 0 (Biomarkers)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Biomarkers/analysis
MH  - Bone Marrow Neoplasms/genetics/*secondary
MH  - Breast Neoplasms/genetics/*pathology
MH  - Carcinoma/genetics/*secondary
MH  - Cell Proliferation
MH  - Disease-Free Survival
MH  - Female
MH  - *Gene Expression Profiling
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Middle Aged
MH  - Mitosis
MH  - *Neoplastic Cells, Circulating
MH  - Prognosis
MH  - Prospective Studies
PMC - PMC1410751
EDAT- 2006/02/07 09:00
MHDA- 2006/02/28 09:00
PMCR- 2005/11/24
CRDT- 2006/02/07 09:00
PHST- 2005/07/31 00:00 [received]
PHST- 2005/10/04 00:00 [revised]
PHST- 2005/10/28 00:00 [accepted]
PHST- 2006/02/07 09:00 [pubmed]
PHST- 2006/02/28 09:00 [medline]
PHST- 2006/02/07 09:00 [entrez]
PHST- 2005/11/24 00:00 [pmc-release]
AID - bcr1360 [pii]
AID - 10.1186/bcr1360 [doi]
PST - ppublish
SO  - Breast Cancer Res. 2005;7(6):R1174-85. doi: 10.1186/bcr1360. Epub 2005 Nov 24.